The transcriptional and functional properties of mouse epiblast stem cells resemble the anterior primitive streak - PubMed (original) (raw)

. 2014 Jan 2;14(1):107-20.

doi: 10.1016/j.stem.2013.09.014. Epub 2013 Oct 17.

Keren Kaufman-Francis 1, Joshua B Studdert 1, Kirsten A Steiner 1, Melinda D Power 1, David A F Loebel 2, Vanessa Jones 1, Angelyn Hor 1, Gustavo de Alencastro 3, Grant J Logan 3, Erdahl T Teber 4, Oliver H Tam 1, Michael D Stutz 5, Ian E Alexander 6, Hilda A Pickett 7, Patrick P L Tam 8

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The transcriptional and functional properties of mouse epiblast stem cells resemble the anterior primitive streak

Yoji Kojima et al. Cell Stem Cell. 2014.

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Abstract

Mouse epiblast stem cells (EpiSCs) can be derived from a wide range of developmental stages. To characterize and compare EpiSCs with different origins, we derived a series of EpiSC lines from pregastrula stage to late-bud-stage mouse embryos. We found that the transcriptomes of these cells are hierarchically distinct from those of the embryonic stem cells, induced pluripotent stem cells (iPSCs), and epiblast/ectoderm. The EpiSCs display globally similar gene expression profiles irrespective of the original developmental stage of the source tissue. They are developmentally similar to the ectoderm of the late-gastrula-stage embryo and behave like anterior primitive streak cells when differentiated in vitro and in vivo. The EpiSC lines that we derived can also be categorized based on a correlation between gene expression signature and predisposition to differentiate into particular germ-layer derivatives. Our findings therefore highlight distinct identifying characteristics of EpiSCs and provide a foundation for further examination of EpiSC properties and potential.

Copyright © 2014 Elsevier Inc. All rights reserved.

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