Absence of the alpha and gamma subunits of 7S nerve growth factor in denervated rodent iris: immunocytochemical studies - PubMed (original) (raw)
Absence of the alpha and gamma subunits of 7S nerve growth factor in denervated rodent iris: immunocytochemical studies
R A Murphy et al. Dev Biol. 1986 Apr.
Abstract
Immunocytochemical studies were performed to determine if denervated rodent iris produces nerve growth factor (NGF) in a form chemically similar to that of the 7S NGF complex in mouse submandibular glands. Antisera to the alpha, beta, and gamma subunits of 7S NGF were raised in rabbits and characterized on immunoblots of SDS-containing polyacrylamide gels. Antisera were applied to stretch preparations of rat and mouse irides that were cultured for periods of 2 to 6 days or sympathetically denervated by superior cervical ganglionectomy and left in situ 4 days. Antibody binding was visualized by indirect immunofluorescence. In control studies done on plastic sections of mouse submandibular glands, antisera co-localized the three subunits of 7S NGF within secretory granules of granular tubule cells. In denervated rat iris, beta NGF immunoreactivity was evident in a cellular plexus that resembled in distribution and morphology nerve fibers in the normal iris, in agreement with a previous study (R.A. Rush (1984). Nature (London) 312, 364-367). Identical staining patterns were observed in mouse iris. In neither rat or mouse, however, did the nerve-like processes stain with antibodies suggests that the NGF-like protein in denervated rodent iris is not synthesized as part of the 7S NGF complex. Iris also did not react with antibodies to epidermal growth factor, a protein co-localized with NGF in mouse submandibular glands and in guinea pig prostate.
Similar articles
- Immunocytochemical localization and concentrations of the alpha and gamma subunits of 7S-nerve growth factor in the submandibular gland of the mouse.
Mowry MD, Jensen RJ, Pantazis NJ. Mowry MD, et al. Cell Tissue Res. 1984;238(3):627-33. doi: 10.1007/BF00219881. Cell Tissue Res. 1984. PMID: 6395962 - Cellular and subcellular colocalization of nerve growth factor and epidermal growth factor in mouse submandibular glands.
Watson AY, Anderson JK, Siminoski K, Mole JE, Murphy RA. Watson AY, et al. Anat Rec. 1985 Nov;213(3):365-76. doi: 10.1002/ar.1092130302. Anat Rec. 1985. PMID: 3907420 - Comparison of 7S nerve growth factor and nerve growth factor I from mouse submandibular glands.
Guerina NG, Pantazis NJ, Siminoski K, Anderson JK, McCarthy M, Stevens CL, Murphy RA. Guerina NG, et al. Biochemistry. 1986 Feb 25;25(4):754-60. doi: 10.1021/bi00352a003. Biochemistry. 1986. PMID: 3964641 - Early days of the nerve growth factor proteins.
Shooter EM. Shooter EM. Annu Rev Neurosci. 2001;24:601-29. doi: 10.1146/annurev.neuro.24.1.601. Annu Rev Neurosci. 2001. PMID: 11283322 Review. - The granular convoluted tubule (GCT) cell of rodent submandibular glands.
Gresik EW. Gresik EW. Microsc Res Tech. 1994 Jan 1;27(1):1-24. doi: 10.1002/jemt.1070270102. Microsc Res Tech. 1994. PMID: 8155902 Review.
Cited by
- Expression of the alpha subunit of 7S nerve growth factor in the mouse submandibular gland.
Isackson PJ, Nisco SJ, Bradshaw RA. Isackson PJ, et al. Neurochem Res. 1987 Oct;12(10):959-66. doi: 10.1007/BF00966319. Neurochem Res. 1987. PMID: 3317101 - Nerve growth factor-like immunoreactivities in rodent salivary glands and testis.
Olson L, Ayer-LeLievre C, Ebendal T, Seiger A. Olson L, et al. Cell Tissue Res. 1987 May;248(2):275-86. doi: 10.1007/BF00218194. Cell Tissue Res. 1987. PMID: 3555833 - Cellular processing of the nerve growth factor precursor by the mammalian pro-protein convertases.
Seidah NG, Benjannet S, Pareek S, Savaria D, Hamelin J, Goulet B, Laliberte J, Lazure C, Chrétien M, Murphy RA. Seidah NG, et al. Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3):951-60. doi: 10.1042/bj3140951. Biochem J. 1996. PMID: 8615794 Free PMC article.