Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli - PubMed (original) (raw)
- PMID: 2423846
- DOI: 10.1007/BF00330395
Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli
S Lin-Chao et al. Mol Gen Genet. 1986 Apr.
Abstract
The concentration of plasmid pBR322, of its replication inhibitor, RNAI, and preprimer, RNAII, were observed in E. coli as functions of the bacterial growth rate. At growth rates between 0.6 and 2.5 doubling/h, the copy number (number of plasmids per genome equivalent of chromosomal DNA) decreased from 32 to 15, the number of plasmids per cell increased from 39 to 55, and the plasmid concentration decreased from 4.6 to 1.1 X 10(10) plasmids per OD460 unit of cell mass. The concentrations of RNAI and RNAII also decreased with increasing growth rate, but differently, such that their ratio, RNAI/RNAII, increased. In glycerol minimal medium both RNAI and RNAII had the same halflife, 0.55 min, and were synthesized at a ratio of about 3 RNAI transcripts per every RNAII transcript. These results were interpreted on the basis of the negative control model and suggest that the activities of the RNAI and RNAII promoters, and the efficiency with which RNAI inhibits plasmid replication, are controlled by the growth rate.
Similar articles
- Activities of the RNAI and RNAII promoters of plasmid pBR322.
Lin-Chao S, Bremer H. Lin-Chao S, et al. J Bacteriol. 1987 Mar;169(3):1217-22. doi: 10.1128/jb.169.3.1217-1222.1987. J Bacteriol. 1987. PMID: 2434459 Free PMC article. - Effect of relA function on the replication of plasmid pBR322 in Escherichia coli.
Lin-Chao S, Bremer H. Lin-Chao S, et al. Mol Gen Genet. 1986 Apr;203(1):150-3. doi: 10.1007/BF00330396. Mol Gen Genet. 1986. PMID: 2423847 - Maintenance of pBR322-derived plasmids without functional RNAI.
Chiang CS, Bremer H. Chiang CS, et al. Plasmid. 1991 Nov;26(3):186-200. doi: 10.1016/0147-619x(91)90042-u. Plasmid. 1991. PMID: 1721720 - Host controlled plasmid replication: Escherichia coli minichromosomes.
Dasgupta S, Løbner-Olesen A. Dasgupta S, et al. Plasmid. 2004 Nov;52(3):151-68. doi: 10.1016/j.plasmid.2004.08.001. Plasmid. 2004. PMID: 15518873 Review. - DNA-protein interactions during replication of genetic elements of bacteria.
Nesvera J, Hochmannová J. Nesvera J, et al. Folia Microbiol (Praha). 1985;30(2):154-76. doi: 10.1007/BF02922209. Folia Microbiol (Praha). 1985. PMID: 2581876 Review.
Cited by
- Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies.
Schmidt M, Lee N, Zhan C, Roberts JB, Nava AA, Keiser LS, Vilchez AA, Chen Y, Petzold CJ, Haushalter RW, Blank LM, Keasling JD. Schmidt M, et al. ACS Synth Biol. 2023 Nov 17;12(11):3366-3380. doi: 10.1021/acssynbio.3c00367. Epub 2023 Oct 18. ACS Synth Biol. 2023. PMID: 37851920 Free PMC article. - Segregational drift hinders the evolution of antibiotic resistance on polyploid replicons.
Garoña A, Santer M, Hülter NF, Uecker H, Dagan T. Garoña A, et al. PLoS Genet. 2023 Aug 3;19(8):e1010829. doi: 10.1371/journal.pgen.1010829. eCollection 2023 Aug. PLoS Genet. 2023. PMID: 37535631 Free PMC article. - High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration.
Elmore JR, Dexter GN, Baldino H, Huenemann JD, Francis R, Peabody GL 5th, Martinez-Baird J, Riley LA, Simmons T, Coleman-Derr D, Guss AM, Egbert RG. Elmore JR, et al. Sci Adv. 2023 Mar 10;9(10):eade1285. doi: 10.1126/sciadv.ade1285. Epub 2023 Mar 10. Sci Adv. 2023. PMID: 36897939 Free PMC article. - A plasmid system with tunable copy number.
Rouches MV, Xu Y, Cortes LBG, Lambert G. Rouches MV, et al. Nat Commun. 2022 Jul 7;13(1):3908. doi: 10.1038/s41467-022-31422-0. Nat Commun. 2022. PMID: 35798738 Free PMC article. - Single cell variability of CRISPR-Cas interference and adaptation.
McKenzie RE, Keizer EM, Vink JNA, van Lopik J, Büke F, Kalkman V, Fleck C, Tans SJ, Brouns SJJ. McKenzie RE, et al. Mol Syst Biol. 2022 Apr;18(4):e10680. doi: 10.15252/msb.202110680. Mol Syst Biol. 2022. PMID: 35467080 Free PMC article.
References
- Plasmid. 1980 Jan;3(1):88-91 - PubMed
- J Theor Biol. 1982 Feb 7;94(3):651-70 - PubMed
- Microbiol Rev. 1983 Jun;47(2):180-230 - PubMed
- Mol Gen Genet. 1986 Apr;203(1):150-3 - PubMed
- Gene. 1984 Apr;28(1):1-15 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources