Murine lupus monoclonal antibodies define five epitopes on two different Sm polypeptides - PubMed (original) (raw)

. 1986 Jul;58(3):495-500.

• PMID: 2426187
• PMCID: PMC1453472

Murine lupus monoclonal antibodies define five epitopes on two different Sm polypeptides

D G Williams et al. Immunology. 1986 Jul.

Abstract

Monoclonal anti-Sm (Smith) antibodies derived from the mouse strain MRL/lpr were isolated and characterized by binding to purified antigen, immunoprecipitating characteristic uridine-rich RNAs from Hela cell extracts, and by Western blot analysis using rabbit thymus extract. Five different Sm epitopes were demonstrated by epitope blockade and probing Western blots with the monoclonal antibodies. Human anti-Sm serum inhibited each monoclonal antibody from binding to antigen, indicating that both human and mouse antibodies bind to the same Sm epitopes. Human anti-Sm antibodies bound to 28,000 and 16,000 MW polypeptides, a small number also binding to a 14,000 MW polypeptide. The monoclonal antibodies also bound to the 28,000 and/or the 16,000 polypeptide, and provided evidence to suggest that these two Sm polypeptides bear some structural similarities, but are distinct molecules.

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References

1. 1. J Immunol. 1973 May;110(5):1318-24 - PubMed
2. 1. J Biol Chem. 1984 Oct 25;259(20):12850-6 - PubMed
3. 1. Ann Intern Med. 1975 Oct;83(4):464-9 - PubMed
4. 1. Arthritis Rheum. 1976 May-Jun;19(3):574-80 - PubMed
5. 1. Eur J Immunol. 1976 Jul;6(7):511-9 - PubMed

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