Interspecific nucleotide sequence comparisons used to identify regulatory and structural features of the Drosophila hsp82 gene - PubMed (original) (raw)
Comparative Study
Interspecific nucleotide sequence comparisons used to identify regulatory and structural features of the Drosophila hsp82 gene
R K Blackman et al. J Mol Biol. 1986.
Abstract
We have sequenced the hsp82 heat shock gene and 5'-flanking DNA of four species of Drosophila in order to identify conserved features of possible regulatory significance and to determine the nature and rate of evolutionary change in various domains of the gene. All conserved sequences identified in the 5' non-transcribed region of the hsp82 gene of melanogaster, simulans, pseudoobscura and virilis lie within 150 base-pairs of the RNA initiation site. These include a 34 base-pair imperfect but highly conserved dyad made up of three overlapping copies of the consensus heat shock regulatory sequence dyad made up of three overlapping copies of the consensus heat shock regulatory sequence C-T-N-G-A-A-N-N-T-T-C-N-A-G. Several other highly conserved features are also seen. In pseudoobscura only, the region from -21 to -133 is almost exactly repeated 698 base-pairs upstream. The upstream repeat retains all of the sequences common to the four species in the -21 to -133 interval except for the T-A-T-A motif. One possibility is that this upstream sequence is involved in dosage compensation of the hsp82 gene, which is pseudoobscura is on the X chromosome. In melanogaster, simulans and virilis there is an oppositely oriented, non-heat shock gene 0.6 to 0.8 kb (kb = 10(3) base-pairs) upstream from the hsp82 transcription initiation site. Comparisons of the hsp82 transcription unit from the distantly related species show little or no sequence conservation in the intron and non-translated exon I sequences. In contrast, the coding regions of these species are 90% homologous at the DNA level and 97 to 99% identical at the amino acid level. Half of the amino acid changes have occurred within a 15-amino acid region that lies within an unusually polar domain of hsp82. From the sequence comparisons, we estimate the rate of silent (synonymous) site substitution in Drosophila as 1 X 10(-8)/nucleotide site per year, similar to that for mammals.
Similar articles
- Dosage compensation of the Drosophila pseudoobscura Hsp82 gene and the Drosophila melanogaster Adh gene at ectopic sites in D. melanogaster.
Sass H, Meselson M. Sass H, et al. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6795-9. doi: 10.1073/pnas.88.15.6795. Proc Natl Acad Sci U S A. 1991. PMID: 1907376 Free PMC article. - Interspecific comparison of a Drosophila gene encoding FMRFamide-related neuropeptides.
Taghert PH, Schneider LE. Taghert PH, et al. J Neurosci. 1990 Jun;10(6):1929-42. doi: 10.1523/JNEUROSCI.10-06-01929.1990. J Neurosci. 1990. PMID: 2113087 Free PMC article. - Heat shock locus 93D of Drosophila melanogaster: a spliced RNA most strongly conserved in the intron sequence.
Garbe JC, Pardue ML. Garbe JC, et al. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1812-6. doi: 10.1073/pnas.83.6.1812. Proc Natl Acad Sci U S A. 1986. PMID: 3081901 Free PMC article. - The 93D heat shock locus of Drosophila melanogaster: modulation by genetic and developmental factors.
Lakhotia SC. Lakhotia SC. Genome. 1989;31(2):677-83. doi: 10.1139/g89-124. Genome. 1989. PMID: 2517262 Review. - The 93D (hsr-omega) locus of Drosophila: non-coding gene with house-keeping functions.
Lakhotia SC, Sharma A. Lakhotia SC, et al. Genetica. 1996 May;97(3):339-48. doi: 10.1007/BF00055320. Genetica. 1996. PMID: 9081862 Review.
Cited by
- Thermal tolerance and heat shock protein synthesis during development in the anuran Lepidobatrachus laevis.
Carroll EJ Jr. Carroll EJ Jr. Dev Growth Differ. 1996 Feb;38(1):9-14. doi: 10.1046/j.1440-169X.1996.00002.x. Dev Growth Differ. 1996. PMID: 37280812 - The polymorphisms in the promoter of HSP90 gene and their association with heat tolerance of bay scallop.
Yang C, Wang L, Liu C, Zhou Z, Zhao X, Song L. Yang C, et al. Cell Stress Chaperones. 2015 Mar;20(2):297-308. doi: 10.1007/s12192-014-0546-z. Epub 2014 Sep 27. Cell Stress Chaperones. 2015. PMID: 25261233 Free PMC article. - cDNA cloning of heat shock protein 90 gene and protein expression pattern in response to heavy metal exposure and thermal stress in planarian Dugesia japonica.
Ma KX, Chen GW, Liu DZ. Ma KX, et al. Mol Biol Rep. 2012 Jun;39(6):7203-10. doi: 10.1007/s11033-012-1552-9. Epub 2012 Mar 6. Mol Biol Rep. 2012. PMID: 22391652 - Molecular characterization and expression of a heat shock protein gene (HSP90) from the carmine spider mite, Tetranychus cinnabarinus (Boisduval).
Feng H, Wang L, Liu Y, He L, Li M, Lu W, Xue C. Feng H, et al. J Insect Sci. 2010;10:112. doi: 10.1673/031.010.11201. J Insect Sci. 2010. PMID: 20874569 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases