Identification of myoepithelial cells in human and rat breasts by anti-common acute lymphoblastic leukemia antigen antibody A12 - PubMed (original) (raw)
- PMID: 2426509
Identification of myoepithelial cells in human and rat breasts by anti-common acute lymphoblastic leukemia antigen antibody A12
B A Gusterson et al. J Natl Cancer Inst. 1986 Aug.
Abstract
An immunohistological study in the human breast and the rodent breast (from inbred Ludwig/Wistar/Olac rats) was conducted with the use of a murine monoclonal antibody, which reacts with the common acute lymphoblastic antigen, a glycosylated polypeptide of a molecular weight of 100,000. The epitope, as recognized by this antibody, is expressed on myoepithelial cells of the normal human and rat breasts and was studied in the developing rodent mammary gland. Ultrastructural studies in the normal human breast clearly demonstrated the presence of the antigen on the lateral membrane of the myoepithelial cells with no staining of luminal cells, blood vessels, or stromal elements. The antigen survived prolonged enzymatic digestion of human breast tissue and could be demonstrated on myoepithelial cells in single-cell suspensions of human breast where it stained approximately 3-14% of the total cell population. The presence of this antigen on myoepithelial cells is discussed in the context of myoepithelial differentiation in the breast and the potential utility of the antibodies for cell separation.
Similar articles
- A novel monoclonal antibody (7B10) with differential reactivity between human mammary carcinoma and normal breast.
Pancino G, Charpin C, Calvo F, Guillemin MC, Roseto A. Pancino G, et al. Cancer Res. 1987 Aug 15;47(16):4444-52. Cancer Res. 1987. PMID: 3607774 - Isolation of pure populations of epithelial and myoepithelial cells from the normal human mammary gland using immunomagnetic separation with Dynabeads.
Gomm JJ, Browne PJ, Coope RC, Liu QY, Buluwela L, Coombes RC. Gomm JJ, et al. Anal Biochem. 1995 Mar 20;226(1):91-9. doi: 10.1006/abio.1995.1196. Anal Biochem. 1995. PMID: 7785785 - Production and characterization of monoclonal antibodies showing a different spectrum of reactivity to human breast tissue.
Mandeville R, Giroux L, Lecomte J, Chausseau JP, Dumas F, Ajdukovic I, Vidal D, Boury F. Mandeville R, et al. Cancer Detect Prev. 1987;10(1-2):89-100. Cancer Detect Prev. 1987. PMID: 2436777 - Growth and differentiation of the normal mammary gland and its tumours.
Rudland PS, Barraclough R, Fernig DG, Smith JA. Rudland PS, et al. Biochem Soc Symp. 1998;63:1-20. Biochem Soc Symp. 1998. PMID: 9513707 Review. - Mammary epithelial stem cells.
Smith GH, Chepko G. Smith GH, et al. Microsc Res Tech. 2001 Jan 15;52(2):190-203. doi: 10.1002/1097-0029(20010115)52:2<190::AID-JEMT1005>3.0.CO;2-O. Microsc Res Tech. 2001. PMID: 11169867 Review.
Cited by
- Activated breast stromal fibroblasts exhibit myoepithelial and mammary stem cells features.
Aboussekhra A, Islam SS, Alraouji NN. Aboussekhra A, et al. Transl Oncol. 2023 Sep;35:101721. doi: 10.1016/j.tranon.2023.101721. Epub 2023 Jun 15. Transl Oncol. 2023. PMID: 37329829 Free PMC article. - Role of neurokinin-1 receptor in the initiation and maintenance of skin chronic inflammatory diseases.
Divito SJ, Morelli AE, Larregina AT. Divito SJ, et al. Immunol Res. 2011 Aug;50(2-3):195-201. doi: 10.1007/s12026-011-8219-9. Immunol Res. 2011. PMID: 21717076 - CD10 expression in urothelial carcinoma of the bladder.
Bahadir B, Behzatoglu K, Bektas S, Bozkurt ER, Ozdamar SO. Bahadir B, et al. Diagn Pathol. 2009 Nov 16;4:38. doi: 10.1186/1746-1596-4-38. Diagn Pathol. 2009. PMID: 19917108 Free PMC article. - Hedgehog signalling in breast cancer.
Kasper M, Jaks V, Fiaschi M, Toftgård R. Kasper M, et al. Carcinogenesis. 2009 Jun;30(6):903-11. doi: 10.1093/carcin/bgp048. Epub 2009 Feb 23. Carcinogenesis. 2009. PMID: 19237605 Free PMC article. Review. - Breast cancer stem cells: implications for therapy of breast cancer.
Morrison BJ, Schmidt CW, Lakhani SR, Reynolds BA, Lopez JA. Morrison BJ, et al. Breast Cancer Res. 2008;10(4):210. doi: 10.1186/bcr2111. Epub 2008 Jul 22. Breast Cancer Res. 2008. PMID: 18671830 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Medical