Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column - PubMed (original) (raw)

Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column

B Vogelstein. Anal Biochem. 1987 Jan.

Abstract

A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors.

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