Properties of the Na+-K+ pump in human red cells with increased number of pump sites - PubMed (original) (raw)

• PMID: 2439543
• PMCID: PMC442210
• DOI: 10.1172/JCI113037

Properties of the Na+-K+ pump in human red cells with increased number of pump sites

J A Halperin et al. J Clin Invest. 1987 Jul.

Abstract

We studied the Na+/K+ pump in red cells from an obese human subject (MAJ) in which the number of pumps/cell was 10-20 times higher than normal. Through measurements of the kinetic properties of several modes of operation of the Na+/K+ pump we determined that the pumps in MAJ cells are kinetically normal. In the presence of adequate metabolic substrate the maximum rates of Na+ pumping and lactate production saturated at 60 and 12 nmol/1 cell per h, respectively. Under physiological conditions pump and "leak" Na+ fluxes were similar in MAJ and normal cells. Since internal Na+ was lower in MAJ than in normal cells (Nai+ approximately 2 and 8 mmol/1 cell, respectively), we conclude that the reduction in cell Na+ allows the Na+/K+ pump in MAJ cells to operate at lower fraction of maximum capacity and to compensate for the increased number of pumps.

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References

1. 1. J Clin Invest. 1967 Jan;46(1):65-76 - PubMed
2. 1. J Clin Invest. 1959 Sep;38:1572-86 - PubMed
3. 1. J Physiol. 1967 Sep;192(1):189-216 - PubMed
4. 1. J Physiol. 1967 Sep;192(1):237-56 - PubMed
5. 1. Am J Med Technol. 1969 Aug;35(8):475-86 - PubMed

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