A human beta-actin expression vector system directs high-level accumulation of antisense transcripts - PubMed (original) (raw)
Comparative Study
A human beta-actin expression vector system directs high-level accumulation of antisense transcripts
P Gunning et al. Proc Natl Acad Sci U S A. 1987 Jul.
Abstract
We have constructed a mammalian expression vector consisting of 3 kilobases of the human beta-actin gene 5' flanking sequence plus 5' untranslated region and intervening sequence 1 linked at the 3' splice site to a short DNA polylinker sequence containing unique Sal I, HindIII, and BamHI restriction endonuclease sites followed by a simian virus 40 (SV40) polyadenylylation signal. Two derivatives, containing the selection markers obtained from pSV2gpt or pSV2neo, were also generated. We find that the promoter activity of this vector is a great or greater than that of the SV40 early promoter in a variety of human and rodent cells. The vector was used to generate gamma-actin and beta-tubulin antisense transcripts in human fibroblast cell lines. The antisense transcripts accumulate to levels comparable with that of the highly abundant gamma-actin and beta-tubulin mRNAs.
References
- J Biol Chem. 1980 Feb 25;255(4):1650-61 - PubMed
- Dev Biol. 1979 Nov;73(1):153-73 - PubMed
- Nucleic Acids Res. 1981 Feb 11;9(3):579-89 - PubMed
- Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6 - PubMed
- J Mol Appl Genet. 1982;1(4):327-41 - PubMed
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