Quantification of trehalose in biological samples with a conidial trehalase from the thermophilic fungus Humicola grisea var. thermoidea - PubMed (original) (raw)

Quantification of trehalose in biological samples with a conidial trehalase from the thermophilic fungus Humicola grisea var. thermoidea

M J Neves et al. World J Microbiol Biotechnol. 1994 Jan.

Abstract

The partially-purified, thermally-stable trehalase from conidia of Humicola grisea was highly specific for trehalose and was free of potentially interfering activities. The enzyme was fully stable when stored in solution at -15°C for at least 6 months. This preparation could be used to quantify trehalose from 0.05 to 1.25 μmol/ml either in carbohydrate mixtures or in complex biological materials.

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References

1. 1. J Bacteriol. 1987 Dec;169(12):5518-22 - PubMed
2. 1. Biochim Biophys Acta. 1990 Mar 26;1033(3):243-9 - PubMed
3. 1. Anal Biochem. 1976 May 7;72:353-8 - PubMed
4. 1. Microbiol Rev. 1984 Mar;48(1):42-59 - PubMed
5. 1. Bull Soc Chim Biol (Paris). 1966;48(2):277-86 - PubMed

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