Identification of a 400-kd protein in the brush borders of human kidney tubules that is similar to gp330, the nephritogenic antigen of rat Heymann nephritis - PubMed (original) (raw)

. 1987 Oct;129(1):183-91.

Affiliations

• PMID: 2444109
• PMCID: PMC1899684

Identification of a 400-kd protein in the brush borders of human kidney tubules that is similar to gp330, the nephritogenic antigen of rat Heymann nephritis

D Kerjaschki et al. Am J Pathol. 1987 Oct.

Abstract

The nephritogenic antigen of Heymann nephritis (HN)--a well-studied experimental rat model disease of human membranous glomerulonephritis (MGN)--was recently shown to be a 330-kd glycoprotein (gp330) which is present in the membranes of both the rat tubular brush borders and of podocytes. Because the pathogenic antigen(s) of MGN are unknown, the authors have searched for a gp330-like molecule in human kidney and for its role in MGN. The authors here report that a membrane protein (apparent molecular weight 400 kd) is present in human kidney which is immunologically cross-reactive with rat gp330. By immunoelectron microscopy (using rabbit anti-rat gp330 IgG or a monoclonal anti-400-kd IgG) this molecule is similarly localized in human proximal tubules, but it is absent from the podocytes of human glomeruli. The 400-kd molecule is not detected in the glomerular immune deposits of 30 biopsies of MGN. It is proposed that this is due to the lack of the 400-kd protein in human glomeruli which prevents the formation of initial 400-kd anti-400-kd IgG immune complexes in situ.

PubMed Disclaimer

Similar articles

• A major pathogenic antigen of Heymann nephritis is present exclusively in the renal proximal tubule brush border--studies with a monoclonal antibody against pronase-digested tubular antigen.
  Tsukada Y, Ono K, Maezawa A, Yano S, Naruse T. Tsukada Y, et al. Clin Exp Immunol. 1994 May;96(2):303-10. doi: 10.1111/j.1365-2249.1994.tb06558.x. Clin Exp Immunol. 1994. PMID: 8187338 Free PMC article.
• Immunocytochemical localization of the Heymann nephritis antigen (GP330) in glomerular epithelial cells of normal Lewis rats.
  Kerjaschki D, Farquhar MG. Kerjaschki D, et al. J Exp Med. 1983 Feb 1;157(2):667-86. doi: 10.1084/jem.157.2.667. J Exp Med. 1983. PMID: 6337231 Free PMC article.
• Detection of two forms of GP330. Their role in Heymann nephritis.
  Bachinsky DR, Zheng G, Niles JL, McLaughlin M, Abbate M, Andres G, Brown D, McCluskey RT. Bachinsky DR, et al. Am J Pathol. 1993 Aug;143(2):598-611. Am J Pathol. 1993. PMID: 8342605 Free PMC article.
• Antigenic targets in epimembranous glomerulonephritis. Experimental data and potential application in human pathology.
  Ronco P, Allegri L, Brianti E, Chatelet F, Van Leer EH, Verroust P. Ronco P, et al. Appl Pathol. 1989;7(2):85-98. Appl Pathol. 1989. PMID: 2567175 Review.
• The Heymann nephritis antigenic complex: megalin (gp330) and RAP.
  Farquhar MG, Saito A, Kerjaschki D, Orlando RA. Farquhar MG, et al. J Am Soc Nephrol. 1995 Jul;6(1):35-47. doi: 10.1681/ASN.V6135. J Am Soc Nephrol. 1995. PMID: 7579068 Review.

Cited by

• Renal cell markers: lighthouses for managing renal diseases.
  Agarwal S, Sudhini YR, Polat OK, Reiser J, Altintas MM. Agarwal S, et al. Am J Physiol Renal Physiol. 2021 Dec 1;321(6):F715-F739. doi: 10.1152/ajprenal.00182.2021. Epub 2021 Oct 11. Am J Physiol Renal Physiol. 2021. PMID: 34632812 Free PMC article.
• Patients with primary membranous nephropathy lack auto-antibodies against LDL receptor, the homologue of megalin in human glomeruli.
  Bruschi M, Candiano G, Murtas C, Prunotto M, Santucci L, Carnevali ML, Scolari F, Allegri L, Ghiggeri GM. Bruschi M, et al. Clin Kidney J. 2012 Apr;5(2):178-179. doi: 10.1093/ndtplus/sfp002. Epub 2012 Jan 31. Clin Kidney J. 2012. PMID: 30305893 Free PMC article. No abstract available.
• LDL Receptor-Related Protein 2 (Megalin) as a Target Antigen in Human Kidney Anti-Brush Border Antibody Disease.
  Larsen CP, Trivin-Avillach C, Coles P, Collins AB, Merchant M, Ma H, Wilkey DW, Ambruzs JM, Messias NC, Cossey LN, Rosales IA, Wooldridge T, Walker PD, Colvin RB, Klein J, Salant DJ, Beck LH Jr. Larsen CP, et al. J Am Soc Nephrol. 2018 Feb;29(2):644-653. doi: 10.1681/ASN.2017060664. Epub 2017 Oct 26. J Am Soc Nephrol. 2018. PMID: 29074737 Free PMC article.
• Significance of urinary full-length megalin in patients with IgA nephropathy.
  Seki T, Asanuma K, Asao R, Nonaka K, Sasaki Y, Oliva Trejo JA, Kurosawa H, Hirayama Y, Horikoshi S, Tomino Y, Saito A. Seki T, et al. PLoS One. 2014 Dec 12;9(12):e114400. doi: 10.1371/journal.pone.0114400. eCollection 2014. PLoS One. 2014. PMID: 25502002 Free PMC article.
• Novel targets for immunotherapy in glomerulonephritis.
  Foster MH. Foster MH. Biologics. 2008 Sep;2(3):531-45. doi: 10.2147/btt.s2764. Biologics. 2008. PMID: 19707383 Free PMC article.

References

1. 1. Eur J Clin Invest. 1984 Feb;14(1):60-6 - PubMed
2. 1. Clin Nephrol. 1981 May;15(5):272-7 - PubMed
3. 1. J Immunol. 1985 Oct;135(4):2400-8 - PubMed
4. 1. J Clin Invest. 1986 Nov;78(5):1142-9 - PubMed
5. 1. J Exp Med. 1968 Mar 1;127(3):555-72 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations