Protective Effects of Lycium barbarum Polysaccharides on Testis Spermatogenic Injury Induced by Bisphenol A in Mice - PubMed (original) (raw)

Protective Effects of Lycium barbarum Polysaccharides on Testis Spermatogenic Injury Induced by Bisphenol A in Mice

Caili Zhang et al. Evid Based Complement Alternat Med. 2013.

Abstract

To observe the effects of Lycium barbarum polysaccharides (LBP) on testis spermatogenic injuries induced by Bisphenol A (BPA) in mice. BPA was subcutaneously injected into mice at a dose of 20 mg/kg body weight (BW) for 7 consecutive days. LBP was administered simultaneously with BPA by gavage daily at the dose of 50, 100, and 200 mg/kg BW for 7 days. After treatment, the weight and the histopathology changes of testis and epididymis were examined; the contents of T, LH, GnRH, antioxidant enzyme, and malondialdehyde (MDA) in serum were detected; proapoptotic protein Bax and antiapoptotic protein Bcl-2 were also detected by immunohistochemical method. Results showed that the weights of testis and epididymis were all increased after supplement with different dosages of LBP compared with BPA group, and the activities of SOD and GSH-Px were significantly increased in LBP groups, while MDA contents were gradually decreased. Moreover, the levels of T, LH, and GnRH were significantly elevated in serum treated with 100 mg/kg LBP. LBP also shows significant positive effects on the expression of Bcl-2/Bax in BPA treated mice. It is concluded that LBP may be one of the potential ingredients protecting the adult male animals from BPA induced reproductive damage.

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Figures

Figure 1

Figure 1

Development of spermatogenic cells in seminiferous tubules of mice testis (Bar = 100 _μ_m). (a) Control group: the histological structure of seminiferous tubules is normal with 5–7 layers of closely and orderly arrayed spermatogenic cells. (b) BPA group: some of the spermatogenic cells desquamate or vanish. The spermatogenic cells array loosely and disorderly. There are gaps between spermospores and primary spermatocytes. (c) 50 mg/kg LBP (L) group: the spermatogenic cells are less closely arrayed, and some of the spermatogenic cells desquamate. (d) 100 mg/kg LBP (M) group: the spermatogenic cells are less closely arrayed and different stages of spermatogenic cells can be identified. (e) 200 mg/kg LBP (H) group: the spermatogenic cells in this group are more closely and tightly arrayed than cells in 50 mg/kg and 100 mg/kg group. The histological structure of seminiferous tubules is clear. The gaps between cells were larger than usual (the thick black arrow); the seminiferous tubules spaces were bigger (the thin black arrow); the spermatogenic cells array loosely and disorderly, and some spermatoggenic cells desquamated (the thick white arrow); the cells array more orderly and closely than other groups except the control group (the thin white arrow).

Figure 2

Figure 2

Effects of LBP on serum levels of T, LH, and GnRH in mice. (a) The content of T in different groups. (b) The content of LH in different groups. (c) The content of GnRH in different groups.

Figure 3

Figure 3

Expression of Bcl-2 and Bax proteins in spermatogenic cells in mice (Bar = 100 _μ_m). (a1)–(e1) Expression of Bax protein. (a2)–(e2) Expression of Bcl-2 protein. (a) Control; (b) BPA group; (c) 50 mg·kg−1 LBP group; (d) 100 mg·kg−1 LBP group; (e) 200 mg·kg−1 LBP group. The thick black arrow indicates spermatogenous cell; the thin black arrow indicates the primary spermatocyte; the white arrow indicates Leydig cell.

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