Metabolomics Reveal d-Alanine:d-Alanine Ligase As the Target of d-Cycloserine in Mycobacterium tuberculosis - PubMed (original) (raw)

Changes in intracellular pool sizes of

d

-alanine pathway metabolites over 8 h, after transfer of H37Rv-laden filters to 7H10 media containing DCS and either (a) 1 mM

l

-Ala-13C-2H or (b) 1 mM

d

-Ala-2H following a 16 h prior exposure to 0× (black), 1× (blue), or 5× (red) MIC of DCS. Metabolite levels followed are

l

-Ala-13C-2H (top left panel), Ala-13C (top middle panel),

d

-Ala-2H (bottom left panel),

d

,

l

-Ala (bottom middle panel), and

d

-Ala:

d

-Ala (top and bottom right panels). _Y_-axis values are relative concentrations of target compounds normalized to the residual protein content of the respective sample. Data are the average ±1 standard deviation of duplicate samples.