ESCRT machinery is required for plasma membrane repair - PubMed (original) (raw)
. 2014 Feb 28;343(6174):1247136.
doi: 10.1126/science.1247136. Epub 2014 Jan 30.
Affiliations
- PMID: 24482116
- DOI: 10.1126/science.1247136
ESCRT machinery is required for plasma membrane repair
Ana Joaquina Jimenez et al. Science. 2014.
Abstract
Plasma membrane damage can be triggered by numerous phenomena, and efficient repair is essential for cell survival. Endocytosis, membrane patching, or extracellular budding can be used for plasma membrane repair. We found that endosomal sorting complex required for transport (ESCRT), involved previously in membrane budding and fission, plays a critical role in plasma membrane repair. ESCRT proteins were recruited within seconds to plasma membrane wounds. Quantitative analysis of wound closure kinetics coupled to mathematical modeling suggested that ESCRTs are involved in the repair of small wounds. Real-time imaging and correlative scanning electron microscopy (SEM) identified extracellular buds and shedding at the site of ESCRT recruitment. Thus, the repair of certain wounds is ensured by ESCRT-mediated extracellular shedding of wounded portions.
Comment in
- Cell biology: ESCRTing trouble out!
McNeil PL. McNeil PL. Curr Biol. 2014 May 5;24(9):R370-2. doi: 10.1016/j.cub.2014.03.044. Curr Biol. 2014. PMID: 24801189
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