Branched pattern of regulatory interactions between late sporulation genes in Bacillus subtilis - PubMed (original) (raw)
Branched pattern of regulatory interactions between late sporulation genes in Bacillus subtilis
J Errington et al. J Bacteriol. 1988 Feb.
Abstract
We measured the synthesis of dipicolinic acid (DPA) during sporulation in spo mutants of Bacillus subtilis by a sensitive biological assay based on cross-feeding of a spoVF mutant strain and also chemically. Many spo mutations, including several that block sporulation at stage III, did not prevent synthesis of DPA but instead prevented its incorporation into the spore. In general, strains with mutations in loci that are expressed in the spore compartment synthesized DPA, whereas strains with mutations in most of the loci that are expressed in the mother-cell compartment did not. Transcription of the gerE gene, as measured by DNA-RNA hybridization, followed a dependence pattern very similar to that of DPA synthesis. However, the dependence patterns of the two operons show that at about stage IV of sporulation there is a branch in the sequence of operon expression in the mother cell. One branch leads through spoVC to synthesis of DPA synthetase, and the other leads through spoVD to expression of gerE.
Similar articles
- The products of the spoVA operon are involved in dipicolinic acid uptake into developing spores of Bacillus subtilis.
Tovar-Rojo F, Chander M, Setlow B, Setlow P. Tovar-Rojo F, et al. J Bacteriol. 2002 Jan;184(2):584-7. doi: 10.1128/JB.184.2.584-587.2002. J Bacteriol. 2002. PMID: 11751839 Free PMC article. - Analysis of the germination of spores of Bacillus subtilis with temperature sensitive spo mutations in the spoVA operon.
Vepachedu VR, Setlow P. Vepachedu VR, et al. FEMS Microbiol Lett. 2004 Oct 1;239(1):71-7. doi: 10.1016/j.femsle.2004.08.022. FEMS Microbiol Lett. 2004. PMID: 15451103 - Dependent sequences of gene expression controlling spore formation in Bacillus subtilis.
Mandelstam J, Errington J. Mandelstam J, et al. Microbiol Sci. 1987 Aug;4(8):238-44. Microbiol Sci. 1987. PMID: 3155276 Review.
Cited by
- Dipicolinic Acid Greatly Enhances Production of Spore Photoproduct in Bacterial Spores upon UV Irradiation.
Setlow B, Setlow P. Setlow B, et al. Appl Environ Microbiol. 1993 Feb;59(2):640-3. doi: 10.1128/aem.59.2.640-643.1993. Appl Environ Microbiol. 1993. PMID: 16348882 Free PMC article. - Role of a SpoVA protein in dipicolinic acid uptake into developing spores of Bacillus subtilis.
Li Y, Davis A, Korza G, Zhang P, Li YQ, Setlow B, Setlow P, Hao B. Li Y, et al. J Bacteriol. 2012 Apr;194(8):1875-84. doi: 10.1128/JB.00062-12. Epub 2012 Feb 10. J Bacteriol. 2012. PMID: 22328679 Free PMC article. - Characterization of Clostridium perfringens spores that lack SpoVA proteins and dipicolinic acid.
Paredes-Sabja D, Setlow B, Setlow P, Sarker MR. Paredes-Sabja D, et al. J Bacteriol. 2008 Jul;190(13):4648-59. doi: 10.1128/JB.00325-08. Epub 2008 May 9. J Bacteriol. 2008. PMID: 18469104 Free PMC article. - Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores.
Sanchez-Salas JL, Setlow P. Sanchez-Salas JL, et al. J Bacteriol. 1993 May;175(9):2568-77. doi: 10.1128/jb.175.9.2568-2577.1993. J Bacteriol. 1993. PMID: 8478323 Free PMC article. - Binding of small, acid-soluble spore proteins to DNA plays a significant role in the resistance of Bacillus subtilis spores to hydrogen peroxide.
Setlow B, Setlow P. Setlow B, et al. Appl Environ Microbiol. 1993 Oct;59(10):3418-23. doi: 10.1128/aem.59.10.3418-3423.1993. Appl Environ Microbiol. 1993. PMID: 8250563 Free PMC article.
References
- J Gen Microbiol. 1987 Mar;133(3):493-502 - PubMed
- J Gen Microbiol. 1972 Jun;71(1):17-27 - PubMed
- J Gen Microbiol. 1986 Nov;132(11):2987-94 - PubMed
- J Bacteriol. 1961 May;81(5):741-6 - PubMed
- J Gen Microbiol. 1987 Sep;133(9):2381-91 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases