Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa - PubMed (original) (raw)

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

H Schägger et al. Anal Biochem. 1987.

Abstract

A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations than in glycine-SDS-PAGE systems. A superior resolution of proteins, especially in the range between 5 and 20 kDa, is achieved without the necessity to use urea. Proteins above 30 kDa are already destacked within the sample gel. Thus a smooth passage of these proteins from sample to separating gel is warranted and overloading effects are reduced. This is of special importance when large amounts of protein are to be loaded onto preparative gels. The omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing.

PubMed Disclaimer

Cited by

New Catalytic Residues and Catalytic Mechanism of the RNase T1 Family.
Takebe K, Suzuki M, Hara Y, Katsutani T, Motoyoshi N, Itagaki T, Miyakawa S, Okamoto K, Fukuzawa K, Kobayashi H. Takebe K, et al. ACS Bio Med Chem Au. 2024 Sep 20;4(5):257-267. doi: 10.1021/acsbiomedchemau.4c00046. eCollection 2024 Oct 16. ACS Bio Med Chem Au. 2024. PMID: 39431265 Free PMC article.
A missense mutation in the barley Xan-h gene encoding the Mg-chelatase subunit I leads to a viable pale green line with reduced daily transpiration rate.
Persello A, Tadini L, Rotasperti L, Ballabio F, Tagliani A, Torricella V, Jahns P, Dalal A, Moshelion M, Camilloni C, Rosignoli S, Hansson M, Cattivelli L, Horner DS, Rossini L, Tondelli A, Salvi S, Pesaresi P. Persello A, et al. Plant Cell Rep. 2024 Sep 29;43(10):246. doi: 10.1007/s00299-024-03328-2. Plant Cell Rep. 2024. PMID: 39343835 Free PMC article.
Haplotype variability in mitochondrial rRNA predisposes to metabolic syndrome.
Pecina P, Čunátová K, Kaplanová V, Puertas-Frias G, Šilhavý J, Tauchmannová K, Vrbacký M, Čajka T, Gahura O, Hlaváčková M, Stránecký V, Kmoch S, Pravenec M, Houštěk J, Mráček T, Pecinová A. Pecina P, et al. Commun Biol. 2024 Sep 11;7(1):1116. doi: 10.1038/s42003-024-06819-w. Commun Biol. 2024. PMID: 39261587 Free PMC article.
Two KaiABC systems control circadian oscillations in one cyanobacterium.
Köbler C, Schmelling NM, Wiegard A, Pawlowski A, Pattanayak GK, Spät P, Scheurer NM, Sebastian KN, Stirba FP, Berwanger LC, Kolkhof P, Maček B, Rust MJ, Axmann IM, Wilde A. Köbler C, et al. Nat Commun. 2024 Sep 3;15(1):7674. doi: 10.1038/s41467-024-51914-5. Nat Commun. 2024. PMID: 39227593 Free PMC article.
Principle Investigation and Method Standardization of Inhibition Zone Assay Based on Antimicrobial Peptides Extracted from Black Soldier Fly Larvae.
Shen W, Xue R, Liu Y, Sun S, Chen X, Sun D, Ouyang H, Li Y, Xu J, Dong X, Ji F, Xu W. Shen W, et al. BioTech (Basel). 2024 Aug 9;13(3):31. doi: 10.3390/biotech13030031. BioTech (Basel). 2024. PMID: 39189210 Free PMC article.

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa - PubMed (original) (raw)