An animal component free medium that promotes the growth of various animal cell lines for the production of viral vaccines - PubMed (original) (raw)

. 2014 May 19;32(24):2767-9.

doi: 10.1016/j.vaccine.2014.02.040. Epub 2014 Feb 26.

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An animal component free medium that promotes the growth of various animal cell lines for the production of viral vaccines

Samia Rourou et al. Vaccine. 2014.

Abstract

IPT-AFM is a proprietary animal component free medium that was developed for rabies virus (strain LP 2061) production in Vero cells. In the present work, we demonstrated the versatility of this medium and its ability to sustain the growth of other cell lines and different virus strains. Here, three models were presented: Vero cells/rabies virus (strain LP 2061), MRC-5 cells/measles virus (strain AIK-C) and BHK-21 cells/rabies virus (strain PV-BHK21). The cell lines were first adapted to grow in IPT-AFM, by progressive reduction of the amount of serum in the culture medium. After their adaptation, BHK-21 cells grew in suspension by forming clumps, whereas MRC-5 cells remained adherent. Then, kinetics of cell growth were studied in agitated cultures for both cell lines. In addition, kinetics of virus replication were investigated.

Keywords: Animal component free medium; BHK-21 cells; MRC-5 cells; Measles viruses; Rabies virus; Vero cells.

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