Human papillomavirus type 16 alters human epithelial cell differentiation in vitro - PubMed (original) (raw)

Human papillomavirus type 16 alters human epithelial cell differentiation in vitro

D J McCance et al. Proc Natl Acad Sci U S A. 1988 Oct.

Abstract

Human papillomavirus (HPV) types 16, 18, 31, and 33 have been implicated as etiologic agents of cervical and penile cancer. Using a cell culture system for keratinocytes which allows stratification and production of differentiation-specific keratins, we have examined the effects of one of these viruses, HPV-16, on the differentiation capabilities of human epithelial cells. A plasmid containing the HPV-16 genome and a neomycin-selectable marker was transfected into primary human epidermal cells and SCC-13 cells, an immortalized squamous cell carcinoma cell line. Cloned neomycin-resistant cell lines were isolated and examined by cell culture on raised collagen rafts. Cell lines containing HPV-16 DNA retained the ability to stratify and express differentiation-specific keratins in the raft system but otherwise failed to differentiate normally. The histological abnormalities induced by HPV-16 closely resembled those seen in genital intraepithelial neoplasia in vivo. Hence, our results support the role of HPV-16 as an etiologic agent in the development of genital neoplasias and suggest a specific system for the study of HPV-16-induced epithelial cancers.

PubMed Disclaimer

References

    1. Cancer Res. 1981 May;41(5):1657-63 - PubMed
    1. Nature. 1985 Oct 17-23;317(6038):639-41 - PubMed
    1. Int J Cancer. 1988 Feb 15;41(2):184-97 - PubMed
    1. Oncogene. 1987;1(3):251-6 - PubMed
    1. Obstet Gynecol. 1987 Apr;69(4):554-62 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources