Human genes for complement components C1r and C1s in a close tail-to-tail arrangement - PubMed (original) (raw)

Human genes for complement components C1r and C1s in a close tail-to-tail arrangement

H Kusumoto et al. Proc Natl Acad Sci U S A. 1988 Oct.

Abstract

Complementary DNA clones for human C1s were isolated from cDNA libraries that were prepared with poly(A)+ RNAs of human liver and HepG2 cells. A clone with the largest cDNA insert of 2664 base pairs (bp) was analyzed for its complete nucleotide sequence. It contained 202 bp of a 5' untranslated region, 45 bp of coding for a signal peptide (15 amino acid residues), 2019 bp for complement component C1s zymogen (673 amino acid residues), 378 bp for a 3' untranslated region, a stop codon, and 17 bp of a poly(A) tail. The amino acid sequence of C1s was 40.5% identical to that of C1r, with excellent matches of tentative disulfide bond locations conserving the overall domain structure of C1r. DNA blotting and sequencing analyses of genomic DNA and of an isolated genomic DNA clone clearly showed that the human genes for C1r and C1s are closely located in a "tail-to-tail" arrangement at a distance of about 9.5 kilobases. Furthermore, RNA blot analyses showed that both C1r and C1s genes are primarily expressed in liver, whereas most other tissues expressed both C1r and C1s genes at much lower levels (less than 10% of that in liver). Multiple molecular sizes of specific mRNAs were observed in the RNA blot analyses for both C1r and C1s, indicating that alternative RNA processing(s), likely an alternative polyadenylylation, might take place for both genes.

PubMed Disclaimer

Cited by

Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease.
Matsushita M, Fujita T. Matsushita M, et al. J Exp Med. 1992 Dec 1;176(6):1497-502. doi: 10.1084/jem.176.6.1497. J Exp Med. 1992. PMID: 1460414 Free PMC article.
Identification of a human non-interferon lymphokine activating monocyte complement biosynthesis.
Drouet C, Reboul A, Colomb M. Drouet C, et al. Biochem J. 1989 Oct 1;263(1):157-64. doi: 10.1042/bj2630157. Biochem J. 1989. PMID: 2481436 Free PMC article.
The scanning model for translation: an update.
Kozak M. Kozak M. J Cell Biol. 1989 Feb;108(2):229-41. doi: 10.1083/jcb.108.2.229. J Cell Biol. 1989. PMID: 2645293 Free PMC article. Review.
Early Components of the Complement Classical Activation Pathway in Human Systemic Autoimmune Diseases.
Lintner KE, Wu YL, Yang Y, Spencer CH, Hauptmann G, Hebert LA, Atkinson JP, Yu CY. Lintner KE, et al. Front Immunol. 2016 Feb 15;7:36. doi: 10.3389/fimmu.2016.00036. eCollection 2016. Front Immunol. 2016. PMID: 26913032 Free PMC article. Review.
Balancing the View of C1q in Transplantation: Consideration of the Beneficial and Detrimental Aspects.
Khedraki R, Noguchi H, Baldwin WM 3rd. Khedraki R, et al. Front Immunol. 2022 Mar 24;13:873479. doi: 10.3389/fimmu.2022.873479. eCollection 2022. Front Immunol. 2022. PMID: 35401517 Free PMC article. No abstract available.

References

1. Nucleic Acids Res. 1981 Feb 25;9(4):879-94 - PubMed
1. Biochemistry. 1987 Dec 29;26(26):8516-24 - PubMed
1. Science. 1982 Apr 16;216(4543):301-3 - PubMed
1. Anal Biochem. 1984 Feb;137(1):266-7 - PubMed
1. Philos Trans R Soc Lond B Biol Sci. 1984 Sep 6;306(1129):293-9 - PubMed

Human genes for complement components C1r and C1s in a close tail-to-tail arrangement - PubMed (original) (raw)