Specific and heritable gene editing in Arabidopsis - PubMed (original) (raw)
Comment
Specific and heritable gene editing in Arabidopsis
Yangbin Gao et al. Proc Natl Acad Sci U S A. 2014.
No abstract available
Conflict of interest statement
The authors declare no conflict of interest.
Figures
Fig. 1.
Efficient production of transgene-free Arabidopsis plants with desired modifications at the targeted loci. Arabidopsis flowers in the T0 generation are infiltrated with Agrobacteria that carry the transgenes for the CRISPR/Cas system and/or a template for modification. T1 plants carrying the transgenes are selected using an appropriate selection marker. Transgene-free plants with the desired modifications (solid blue colored seedling without a purple circle) can be obtained in the T2 generation. A purple circle indicates seeds, seedlings, or flowers that carry transgenes in the genome. Green, blue, red, and yellow refer to the cells that carry WT copy, desired modification, mutation 1, and mutation 2 at the targeted gene locus, respectively. There can be additional mutations in the population that are not shown in the figure. Solid colors indicate homozygous for the particular alleles. Stripped colors refer to heterozygous for the particular gene.
Comment on
- Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis.
Feng Z, Mao Y, Xu N, Zhang B, Wei P, Yang DL, Wang Z, Zhang Z, Zheng R, Yang L, Zeng L, Liu X, Zhu JK. Feng Z, et al. Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):4632-7. doi: 10.1073/pnas.1400822111. Epub 2014 Feb 18. Proc Natl Acad Sci U S A. 2014. PMID: 24550464 Free PMC article.
References
- Shan Q, et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013;31(8):686–688. - PubMed
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