Omega-3 fatty acids protect renal functions by increasing docosahexaenoic acid-derived metabolite levels in SHR.Cg-Lepr(cp)/NDmcr rats, a metabolic syndrome model - PubMed (original) (raw)

Omega-3 fatty acids protect renal functions by increasing docosahexaenoic acid-derived metabolite levels in SHR.Cg-Lepr(cp)/NDmcr rats, a metabolic syndrome model

Masanori Katakura et al. Molecules. 2014.

Abstract

The omega-3 polyunsaturated fatty acids (ω-3 PUFAs) docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA) protect against diabetic nephropathy by inhibiting inflammation. The aim of this study was to assess the effects of highly purified DHA and EPA or EPA only administration on renal function and renal eicosanoid and docosanoid levels in an animal model of metabolic syndrome, SHR.Cg-Lepr(cp)/NDmcr (SHRcp) rats. Male SHRcp rats were divided into 3 groups. Control (5% arabic gum), TAK-085 (300 mg/kg/day, containing 467 mg/g EPA and 365 mg/g DHA), or EPA (300 mg/kg/day) was orally administered for 20 weeks. The urinary albumin to creatinine ratio in the TAK-085-administered group was significantly lower than that in other groups. The glomerular sclerosis score in the TAK-085-administered group was significantly lower than that in the other groups. Although DHA levels were increased in total kidney fatty acids, the levels of nonesterified DHA were not significantly different among the 3 groups, whereas the levels of protectin D1, resolvin D1, and resolvin D2 were significantly increased in the TAK-085-administered group. The results show that the use of combination therapy with DHA and EPA in SHRcp rats improved or prevented renal failure associate with metabolic syndrome with decreasing triglyceride levels and increasing ω-3 PUFA lipid mediators.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1

Figure 1

Photomicrographs of coronal sections of the glomeruli from (a) the control group, (b) the TAK-085-administered group, (c) and the EPA-administered group. (d) Glomerulosclerosis was semi-quantitatively evaluated. Values are presented as mean ± SE (n = 10–11). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s test. * P < 0.05 vs. control group.

Figure 2

Figure 2

Effects of TAK-085 and EPA on lipid levels in the kidney and liver. TG (a) and TC (b) levels in the kidney. TG (c) and TC (d) levels in the liver. Values are presented as mean ± SE (n = 10–11). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s test. * P < 0.05 vs. control group.

Figure 3

Figure 3

Renal levels of ARA-derived eicosanoids. (a) Nonesterified ARA, (b) PGE2, (c) PGD2, (d) PGF2α, (e) 5-HETE, (f) 12-HETE, and (g) 15-HETE. Values are presented as mean ± SE (n = 10–11). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s test. * P < 0.05 vs. control group.

Figure 4

Figure 4

Renal levels of EPA-derived eicosanoids. (a) Nonesterified EPA, (b) 5-HEPE, (c) 12-HEPE, (d) 15-HEPE, (e) 18-HEPE, (f) RvE2, and (g) RvE3. Values are presented as mean ± SE (n = 10–11). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s test. * P < 0.05 vs. control group.

Figure 5

Figure 5

Renal levels of DHA-derived docosanoids. (a) Nonesterified DHA, (b) 7-HDoHE, (c) 13-HDoHE, (d) 10-HDoHE, (e) 14-HDoHE, (f) 17-HDoHE, (g) PD1, (h) RvD1, and (i) RvD2. Values are presented as mean ± SE (n = 10–11). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s test. * P < 0.05 vs. control group.

Similar articles

Cited by

References

    1. Kielar M.L., Jeyarajah D.R., Zhou X.J., Lu C.Y. Docosahexaenoic Acid Ameliorates Murine Ischemic Acute Renal Failure and Prevents Increases in mRNA Abundance for both TNF-Alpha and Inducible Nitric Oxide Synthase. J. Am. Soc. Nephrol. 2003;14:389–396. doi: 10.1097/01.ASN.0000045047.44107.0B. - DOI - PubMed
    1. Jia Q., Zhou H.R., Bennink M., Pestka J.J. Docosahexaenoic Acid Attenuates Mycotoxin-Induced Immunoglobulin a Nephropathy, Interleukin-6 Transcription, and Mitogen-Activated Protein Kinase Phosphorylation in Mice. J. Nutr. 2004;134:3343–3349. - PubMed
    1. Jia Q., Shi Y., Bennink M.B., Pestka J.J. Docosahexaenoic Acid and Eicosapentaenoic Acid, but Not Alpha-Linolenic Acid, Suppress Deoxynivalenol-Induced Experimental IgA Nephropathy in Mice. J. Nutr. 2004;134:1353–1361. - PubMed
    1. Parinyasiri U., Ong-Ajyooth L., Parichatikanond P., Ong-Ajyooth S., Liammongkolkul S., Kanyog S. Effect of Fish Oil on Oxidative Stress, Lipid Profile and Renal Function in IgA Nephropathy. J. Med. Assoc. Thai. 2004;87:143–149. - PubMed
    1. Mariee A.D., Abd-Ellah M.F. Protective Effect of Docosahexaenoic Acid Against Cyclosporine A-Induced Nephrotoxicity in Rats: A Possible Mechanism of Action. Ren. Fail. 2011;33:66–71. doi: 10.3109/0886022X.2010.541584. - DOI - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources