PADGEM (GMP140) is a component of Weibel-Palade bodies of human endothelial cells - PubMed (original) (raw)
. 1989 Apr;73(5):1109-12.
Affiliations
- PMID: 2467701
Free article
PADGEM (GMP140) is a component of Weibel-Palade bodies of human endothelial cells
R Bonfanti et al. Blood. 1989 Apr.
Free article
Abstract
PADGEM protein (PADGEM), also known as GMP140, is a platelet alpha-granule membrane protein that is translocated to the external membrane after platelet activation. Although the biosynthesis of this protein was originally thought to be confined to megakaryocytes, the synthesis of PADGEM in endothelial cells was recently demonstrated (McEver et al: Blood 70:1974a, 1987). We now describe the subcellular localization of this protein in endothelial cells. Immunofluorescence staining of permeabilized human umbilical vein endothelial cells with KC4, a well characterized monoclonal antibody to PADGEM, showed positively stained elongated structures similar in distribution and shape to Weibel-Palade bodies. Their identity as Weibel-Palade bodies was confirmed by double label immunofluorescence using KC4 and a polyclonal antiserum to von Willebrand factor (vWf), a protein known to be specifically stored in these organelles. All Weibel-Palade bodies were found to contain PADGEM. In contrast to strong perinuclear staining produced with anti-vWf antibodies, no significant perinuclear staining was obtained with KC4, indicating that relatively little PADGEM is present in the endoplasmic reticulum and in the Golgi apparatus. In endothelial cells treated with secretagogues that stimulate vWf release the elongated structures positive for PADGEM disappeared, further identifying these structures as Weibel-Palade bodies. This observation extends the parallels between Weibel-Palade bodies and alpha-granules and suggests a possible functional association between vWf and PADGEM.
Similar articles
- CD63 is a component of Weibel-Palade bodies of human endothelial cells.
Vischer UM, Wagner DD. Vischer UM, et al. Blood. 1993 Aug 15;82(4):1184-91. Blood. 1993. PMID: 8353283 - Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells.
Wagner DD, Olmsted JB, Marder VJ. Wagner DD, et al. J Cell Biol. 1982 Oct;95(1):355-60. doi: 10.1083/jcb.95.1.355. J Cell Biol. 1982. PMID: 6754744 Free PMC article. - [Weibel-Palade bodies: unique secretory organelles within endothelial cells].
Romani de Wit T, Rondaij MG, van Mourik JA. Romani de Wit T, et al. Ned Tijdschr Geneeskd. 2004 Aug 7;148(32):1572-7. Ned Tijdschr Geneeskd. 2004. PMID: 15382555 Review. Dutch. - Orchestration of Primary Hemostasis by Platelet and Endothelial Lysosome-Related Organelles.
Karampini E, Bierings R, Voorberg J. Karampini E, et al. Arterioscler Thromb Vasc Biol. 2020 Jun;40(6):1441-1453. doi: 10.1161/ATVBAHA.120.314245. Epub 2020 May 7. Arterioscler Thromb Vasc Biol. 2020. PMID: 32375545 Review.
Cited by
- Saponins from Allium macrostemon Bulbs Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway.
Liu L, Qiu L, Xue J, Zhong C, Qin M, Zhang Y, Xu C, Xie Y, Yu J. Liu L, et al. Molecules. 2024 Mar 11;29(6):1239. doi: 10.3390/molecules29061239. Molecules. 2024. PMID: 38542876 Free PMC article. - Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase.
Naß J, Terglane J, Zeuschner D, Gerke V. Naß J, et al. Adv Sci (Weinh). 2024 Apr;11(16):e2306624. doi: 10.1002/advs.202306624. Epub 2024 Feb 15. Adv Sci (Weinh). 2024. PMID: 38359017 Free PMC article. - Lung endothelium, tau, and amyloids in health and disease.
Balczon R, Lin MT, Voth S, Nelson AR, Schupp JC, Wagener BM, Pittet JF, Stevens T. Balczon R, et al. Physiol Rev. 2024 Apr 1;104(2):533-587. doi: 10.1152/physrev.00006.2023. Epub 2023 Aug 10. Physiol Rev. 2024. PMID: 37561137 Free PMC article. Review. - Heparin, Heparan Sulphate and Sepsis: Potential New Options for Treatment.
Hogwood J, Gray E, Mulloy B. Hogwood J, et al. Pharmaceuticals (Basel). 2023 Feb 10;16(2):271. doi: 10.3390/ph16020271. Pharmaceuticals (Basel). 2023. PMID: 37259415 Free PMC article. Review. - α1-Adrenergic Stimulation Increases Platelet Adhesion to Endothelial Cells Mediated by TRPC6.
Llancalahuen FM, Vallejos A, Aravena D, Prado Y, Gatica S, Otero C, Simon F. Llancalahuen FM, et al. Adv Exp Med Biol. 2023;1408:65-82. doi: 10.1007/978-3-031-26163-3_4. Adv Exp Med Biol. 2023. PMID: 37093422
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous