Osteoclast-like cells formed in long-term human bone marrow cultures express a similar surface phenotype as authentic osteoclasts - PubMed (original) (raw)
Affiliations
- PMID: 2468824
Osteoclast-like cells formed in long-term human bone marrow cultures express a similar surface phenotype as authentic osteoclasts
T Kukita et al. Lab Invest. 1989 Apr.
Abstract
Long-term cultures of human bone marrow form multinucleated cells (MNC) with many functional characteristics of osteoclasts including: expression of tartrate-resistant acid phosphatase, appropriate responses to osteotropic hormones, calcitonin-induced contraction and formation of resorption lacunae on calcified matrices. However, it is unclear if these cells express similar surface antigens as expressed by authentic osteoclasts, since they form on plastic surfaces in the absence of bone. Bone may be required to complete the differentiation process for osteoclasts. Therefore, we have examined the surface phenotype of MNC and compared it with that of osteoclasts freshly isolated from bone, to determine if MNC express similar surface antigens, and if MNC express antigens which identify their cellular origin. Similar to bone-derived osteoclasts, MNC formed in long-term human bone marrow culture expressed osteoclast-specific antigens (detected by monoclonal antibodies 13c2 and 23c6) and did not express Fc receptors, T cell specific antigens, most myeloid antigens or mature macrophage antigens. In contrast to authentic osteoclasts, MNC reacted with a monoclonal antibody (Mol) which identifies an antigen present on myeloblasts, monocytes, granulocytes, and null cells from human peripheral blood and bone marrow. MNC also reacted with the monoclonal antibody My11, which is present on CFU-GM, the granulocyte-macrophage colony-forming cell, the probable precursor for MNC. These data demonstrate that MNC formed in long-term human marrow cultures express a similar surface phenotype to osteoclasts. This phenotype is different from that expressed by macrophage polykaryons. In addition, MNC also expressed monocyte-related antigens (My11, Mol), suggesting that are derived from or related to the monocytic lineage.
Similar articles
- Osteoclast-like cell formation in fetal and newborn long-term baboon marrow cultures is more sensitive to 1,25-dihydroxyvitamin D3 than adult long-term marrow cultures.
Takahashi N, Mundy GR, Kuehl TJ, Roodman GD. Takahashi N, et al. J Bone Miner Res. 1987 Aug;2(4):311-7. doi: 10.1002/jbmr.5650020408. J Bone Miner Res. 1987. PMID: 3455616 - Effects of stem cell factor on osteoclast-like cell formation in long-term human marrow cultures.
Demulder A, Suggs SV, Zsebo KM, Scarcez T, Roodman GD. Demulder A, et al. J Bone Miner Res. 1992 Nov;7(11):1337-44. doi: 10.1002/jbmr.5650071114. J Bone Miner Res. 1992. PMID: 1281606 - IL-6 stimulates osteoclast-like multinucleated cell formation in long term human marrow cultures by inducing IL-1 release.
Kurihara N, Bertolini D, Suda T, Akiyama Y, Roodman GD. Kurihara N, et al. J Immunol. 1990 Jun 1;144(11):4226-30. J Immunol. 1990. PMID: 2341718 - Osteoclasts: structure and function.
Pierce AM, Lindskog S, Hammarström L. Pierce AM, et al. Electron Microsc Rev. 1991;4(1):1-45. doi: 10.1016/0892-0354(91)90015-5. Electron Microsc Rev. 1991. PMID: 1873485 Review. - Osteoclast development: the cell surface and the bone environment.
Osdoby P, Oursler MJ, Salino-Hugg T, Krukowski M. Osdoby P, et al. Ciba Found Symp. 1988;136:108-24. doi: 10.1002/9780470513637.ch8. Ciba Found Symp. 1988. PMID: 3068005 Review.
Cited by
- Annexin II increases osteoclast formation by stimulating the proliferation of osteoclast precursors in human marrow cultures.
Menaa C, Devlin RD, Reddy SV, Gazitt Y, Choi SJ, Roodman GD. Menaa C, et al. J Clin Invest. 1999 Jun;103(11):1605-13. doi: 10.1172/JCI6374. J Clin Invest. 1999. PMID: 10359570 Free PMC article. - A sequential culture approach to study osteoclast differentiation from nonadherent porcine bone marrow cells.
Scheven BA, Milne JS, Robins SP. Scheven BA, et al. In Vitro Cell Dev Biol Anim. 1998 Jul-Aug;34(7):568-77. doi: 10.1007/s11626-998-0117-7. In Vitro Cell Dev Biol Anim. 1998. PMID: 9719417 - Downregulation of calcitonin receptor mRNA expression by calcitonin during human osteoclast-like cell differentiation.
Takahashi S, Goldring S, Katz M, Hilsenbeck S, Williams R, Roodman GD. Takahashi S, et al. J Clin Invest. 1995 Jan;95(1):167-71. doi: 10.1172/JCI117634. J Clin Invest. 1995. PMID: 7814611 Free PMC article. - The role of 1,25-dihydroxycholecalciferol and prostaglandin E2 in the regulation of human osteoclastic bone resorption in vitro.
Flanagan AM, Stow MD, Kendall N, Brace W. Flanagan AM, et al. Int J Exp Pathol. 1995 Feb;76(1):37-42. Int J Exp Pathol. 1995. PMID: 7734338 Free PMC article. - Osteoinductive factor inhibits formation of human osteoclast-like cells.
Kukita A, Bonewald L, Rosen D, Seyedin S, Mundy GR, Roodman GD. Kukita A, et al. Proc Natl Acad Sci U S A. 1990 Apr;87(8):3023-6. doi: 10.1073/pnas.87.8.3023. Proc Natl Acad Sci U S A. 1990. PMID: 2326263 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources