HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA - PubMed (original) (raw)
HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA
C Barat et al. EMBO J. 1989 Nov.
Abstract
The virion cores of the replication competent type 1 human immunodeficiency virus (HIV-1), a retrovirus, contain and RNA genome associated with nucleocapsid (NC) and reverse transcriptase (RT p66/p51) molecules. In vitro reconstructions of these complexes with purified components show that NC is required for efficient annealing of the primer tRNALys,3. In the absence of NC, HIV-1 RT is unable to retrotranscribe the viral RNA template from the tRNA primer. We demonstrate that the HIV-1 RT p66/p51 specifically binds to its cognate primer tRNALys,3 even in the presence of a 100-fold molar excess of other tRNAs. Cross-linking analysis of this interaction locates the contact site to a region within the heavily modified anti-codon domain of tRNALys,3.
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References
- Proc Natl Acad Sci U S A. 1986 Oct;83(20):7648-52 - PubMed
- Biochimie. 1976;58(10):1159-65 - PubMed
- J Virol. 1986 Nov;60(2):771-5 - PubMed
- Virology. 1987 Jan;156(1):171-6 - PubMed
- J Biol Chem. 1987 Apr 15;262(11):4961-7 - PubMed
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