Long-term culture and fine specificity of human cytotoxic T-lymphocyte clones reactive with human immunodeficiency virus type 1 - PubMed (original) (raw)
Long-term culture and fine specificity of human cytotoxic T-lymphocyte clones reactive with human immunodeficiency virus type 1
B D Walker et al. Proc Natl Acad Sci U S A. 1989 Dec.
Abstract
The definition of human immunodeficiency virus type 1 (HIV-1) immunogenic epitopes is central to the rational design of AIDS vaccine strategies. In this study, we have generated seven HIV-1 reverse transcriptase-specific cytotoxic T-lymphocyte (CTL) clones from the peripheral blood of two seropositive subjects. Epitopes recognized by these CTL clones were identified by using target cells infected with recombinant HIV-1-vaccinia virus vectors expressing truncated reverse transcriptase proteins and further defined by using target cells incubated with overlapping 25-amino acid synthetic reverse transcriptase peptides. Five different CTL epitopes were identified, and in each case recognition was restricted by class I human leukocyte antigens (HLA). Clones maintained specific cytolytic function in continuous culture for up to 11 months, requiring only periodic restimulation with a CD3-specific monoclonal antibody. These results indicate that HIV-1-specific, major histocompatibility class I-restricted CTL recognize multiple epitopes of a single viral gene product in conjunction with different host HLA antigens. In addition, they demonstrate that human virus-specific CTL can be grown in long-term culture without the need for reexposure to viral antigen.
Similar articles
- An epitope in human immunodeficiency virus 1 reverse transcriptase recognized by both mouse and human cytotoxic T lymphocytes.
Hosmalin A, Clerici M, Houghten R, Pendleton CD, Flexner C, Lucey DR, Moss B, Germain RN, Shearer GM, Berzofsky JA. Hosmalin A, et al. Proc Natl Acad Sci U S A. 1990 Mar;87(6):2344-8. doi: 10.1073/pnas.87.6.2344. Proc Natl Acad Sci U S A. 1990. PMID: 1690429 Free PMC article. - Human immunodeficiency virus type 1-specific cytotoxic T lymphocytes release gamma interferon, tumor necrosis factor alpha (TNF-alpha), and TNF-beta when they encounter their target antigens.
Jassoy C, Harrer T, Rosenthal T, Navia BA, Worth J, Johnson RP, Walker BD. Jassoy C, et al. J Virol. 1993 May;67(5):2844-52. doi: 10.1128/JVI.67.5.2844-2852.1993. J Virol. 1993. PMID: 7682629 Free PMC article. - Gag-specific cytotoxic T lymphocytes from human immunodeficiency virus type 1-infected individuals: Gag epitopes are clustered in three regions of the p24gag protein.
Buseyne F, McChesney M, Porrot F, Kovarik S, Guy B, Rivière Y. Buseyne F, et al. J Virol. 1993 Feb;67(2):694-702. doi: 10.1128/JVI.67.2.694-702.1993. J Virol. 1993. PMID: 7678303 Free PMC article. - Comprehensive screening for human immunodeficiency virus type 1 subtype-specific CD8 cytotoxic T lymphocytes and definition of degenerate epitopes restricted by HLA-A0207 and -C(W)0304 alleles.
Currier JR, deSouza M, Chanbancherd P, Bernstein W, Birx DL, Cox JH. Currier JR, et al. J Virol. 2002 May;76(10):4971-86. doi: 10.1128/jvi.76.10.4971-4986.2002. J Virol. 2002. PMID: 11967314 Free PMC article.
Cited by
- Polymorphic residues in HLA-B that mediate HIV control distinctly modulate peptide interactions with both TCR and KIR molecules.
Tano-Menka R, Singh NK, Muzhingi I, Li X, Mandanas MV, Kaseke C, Crain CR, Zhang A, Ogunshola FJ, Vecchiarello L, Piechocka-Trocha A, Bashirova A, Birnbaum ME, Carrington M, Walker BD, Gaiha GD. Tano-Menka R, et al. Structure. 2024 Aug 8;32(8):1121-1136.e5. doi: 10.1016/j.str.2024.04.015. Epub 2024 May 10. Structure. 2024. PMID: 38733995 Free PMC article. - Molecular basis of differential HLA class I-restricted T cell recognition of a highly networked HIV peptide.
Li X, Singh NK, Collins DR, Ng R, Zhang A, Lamothe-Molina PA, Shahinian P, Xu S, Tan K, Piechocka-Trocha A, Urbach JM, Weber JK, Gaiha GD, Takou Mbah OC, Huynh T, Cheever S, Chen J, Birnbaum M, Zhou R, Walker BD, Wang JH. Li X, et al. Nat Commun. 2023 May 22;14(1):2929. doi: 10.1038/s41467-023-38573-8. Nat Commun. 2023. PMID: 37217466 Free PMC article. - Codon optimization and improved delivery/immunization regimen enhance the immune response against wild-type and drug-resistant HIV-1 reverse transcriptase, preserving its Th2-polarity.
Latanova AA, Petkov S, Kilpelainen A, Jansons J, Latyshev OE, Kuzmenko YV, Hinkula J, Abakumov MA, Valuev-Elliston VT, Gomelsky M, Karpov VL, Chiodi F, Wahren B, Logunov DY, Starodubova ES, Isaguliants MG. Latanova AA, et al. Sci Rep. 2018 May 24;8(1):8078. doi: 10.1038/s41598-018-26281-z. Sci Rep. 2018. PMID: 29799015 Free PMC article. - T-Cell Receptor (TCR) Clonotype-Specific Differences in Inhibitory Activity of HIV-1 Cytotoxic T-Cell Clones Is Not Mediated by TCR Alone.
Flerin NC, Chen H, Glover TD, Lamothe PA, Zheng JH, Fang JW, Ndhlovu ZM, Newell EW, Davis MM, Walker BD, Goldstein H. Flerin NC, et al. J Virol. 2017 Feb 28;91(6):e02412-16. doi: 10.1128/JVI.02412-16. Print 2017 Mar 15. J Virol. 2017. PMID: 28077649 Free PMC article. - HIV-1 Vpu Mediates HLA-C Downregulation.
Apps R, Del Prete GQ, Chatterjee P, Lara A, Brumme ZL, Brockman MA, Neil S, Pickering S, Schneider DK, Piechocka-Trocha A, Walker BD, Thomas R, Shaw GM, Hahn BH, Keele BF, Lifson JD, Carrington M. Apps R, et al. Cell Host Microbe. 2016 May 11;19(5):686-95. doi: 10.1016/j.chom.2016.04.005. Cell Host Microbe. 2016. PMID: 27173934 Free PMC article.
References
- Cell. 1986 Mar 28;44(6):959-68 - PubMed
- Antimicrob Agents Chemother. 1989 Jan;33(1):53-7 - PubMed
- Science. 1986 Nov 7;234(4777):728-31 - PubMed
- Nature. 1987 Jul 23-29;328(6128):345-8 - PubMed
- Nature. 1987 Jul 23-29;328(6128):348-51 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials