Synthesis of alpha-fetoprotein in hepatocytes is co-ordinately regulated with cell-cell and cell-matrix interactions - PubMed (original) (raw)
Affiliations
- PMID: 2482404
Synthesis of alpha-fetoprotein in hepatocytes is co-ordinately regulated with cell-cell and cell-matrix interactions
A S Gleiberman et al. Mol Biol Med. 1989 Apr.
Abstract
The synthesis of alpha-fetoprotein in primary cultures of adult mouse hepatocytes was studied under different conditions of cultivation. On dried collagen, the majority of cells resumed synthesis of alpha-fetoprotein. Alpha-fetoprotein re-expression was accompanied by the flattening of hepatocytes, loss of gap junctional communication and domain-specific membrane antigens, and by alteration of actin pattern. Cultivation of hepatocytes on polymerized collagen gel did not prevent the re-expression of alpha-fetoprotein and other alterations described above. Hepatocyte cultivation within three-dimensional collagen gel as well as co-cultivation of hepatocytes with liver epithelial cells IAR-20 led to re-establishment of organotypic trabeculae-like structures consisting of polygonal hepatocytes with normal pattern of actin, membrane antigens and cell-cell communication. These structures were almost alpha-fetoprotein-negative. It is suggested that hepatocyte shape is co-ordinately regulated with the switch from an adult "block" of hepatocyte functions to an embryonic one. Three-dimensional extracellular matrix may in turn, influence the maintenance of hepatocyte shape.
Similar articles
- [Synthesis of alpha-fetoprotein in primary cultures of hepatocytes from adult mice].
Gleĭberman AS. Gleĭberman AS. Biull Eksp Biol Med. 1982 Jan;93(1):55-8. Biull Eksp Biol Med. 1982. PMID: 6175362 Russian. - Requirement of 3D extracellular network for maintenance of mature hepatocyte morphology and suppression of alpha-fetoprotein synthesis in vitro.
Kudryavtseva EI, Engelhardt NV. Kudryavtseva EI, et al. Immunol Lett. 2003 Nov 15;90(1):25-31. doi: 10.1016/s0165-2478(03)00162-7. Immunol Lett. 2003. PMID: 14611904 - [Ontogeny of the mouse liver studied with hepatocyte membrane antigens and a comparison with alpha-fetoprotein synthesis].
Shipova LIa, Kuprina NI, Rudinskaia TD. Shipova LIa, et al. Ontogenez. 1988 May-Jun;19(3):264-9. Ontogenez. 1988. PMID: 2459640 Russian. - Extracellular matrix regulation of cell-cell communication and tissue-specific gene expression in primary liver cultures.
Fujita M, Spray DC, Choi H, Saez J, Jefferson DM, Hertzberg E, Rosenberg LC, Reid LM. Fujita M, et al. Prog Clin Biol Res. 1986;226:333-60. Prog Clin Biol Res. 1986. PMID: 3543962 Review.
Cited by
- Regulation of somatic growth by the p160 coactivator p/CIP.
Wang Z, Rose DW, Hermanson O, Liu F, Herman T, Wu W, Szeto D, Gleiberman A, Krones A, Pratt K, Rosenfeld R, Glass CK, Rosenfeld MG. Wang Z, et al. Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13549-54. doi: 10.1073/pnas.260463097. Proc Natl Acad Sci U S A. 2000. PMID: 11087842 Free PMC article. - Liver cell models in in vitro toxicology.
Guillouzo A. Guillouzo A. Environ Health Perspect. 1998 Apr;106 Suppl 2(Suppl 2):511-32. doi: 10.1289/ehp.98106511. Environ Health Perspect. 1998. PMID: 9599700 Free PMC article. Review. - Modeling the growth and morphogenesis of malignant tumors.
Slepkov VA, Sukhovolskii VG, Khlebopros RG. Slepkov VA, et al. Dokl Biochem Biophys. 2006 Nov-Dec;411:342-5. doi: 10.1134/s1607672906060056. Dokl Biochem Biophys. 2006. PMID: 17396577 No abstract available. - Pancreatic expression of keratinocyte growth factor leads to differentiation of islet hepatocytes and proliferation of duct cells.
Krakowski ML, Kritzik MR, Jones EM, Krahl T, Lee J, Arnush M, Gu D, Sarvetnick N. Krakowski ML, et al. Am J Pathol. 1999 Mar;154(3):683-91. doi: 10.1016/S0002-9440(10)65315-1. Am J Pathol. 1999. PMID: 10079246 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Miscellaneous