Hsp70 chaperones are non-equilibrium machines that achieve ultra-affinity by energy consumption - PubMed (original) (raw)
Hsp70 chaperones are non-equilibrium machines that achieve ultra-affinity by energy consumption
Paolo De Los Rios et al. Elife. 2014.
Abstract
70-kDa Heat shock proteins are ATP-driven molecular chaperones that perform a myriad of essential cellular tasks. Although structural and biochemical studies have shed some light on their functional mechanism, the fundamental issue of the role of energy consumption, due to ATP-hydrolysis, has remained unaddressed. Here we establish a clear connection between the non-equilibrium nature of Hsp70, due to ATP hydrolysis, and the determining feature of its function, namely its high affinity for its substrates. Energy consumption can indeed decrease the dissociation constant of the chaperone-substrate complex by several orders of magnitude with respect to an equilibrium scenario. We find that the biochemical requirements for observing such ultra-affinity coincide with the physiological conditions in the cell. Our results rationalize several experimental observations and pave the way for further analysis of non-equilibrium effects underlying chaperone functions.DOI: http://dx.doi.org/10.7554/eLife.02218.001\.
Keywords: chaperones; dissociation constant; non-equilibrium.
Copyright © 2014, De Los Rios and Barducci.
Conflict of interest statement
The authors declare that no competing interests exist.
Figures
Figure 1.. Canonical Hsp70 biochemical cycle.
The model takes into account four states in Hsp70 (NBD is schematically represented here in green, SBD in orange), which are defined by substrate binding and by the nature of the bound nucleotide (ADP or ATP). The rates of the substrate binding/unbinding process (horizontal blue lines) are influenced by the nucleotide (kATPon_,_ kATPoff_,_ kATPon_,_ kATPoff). ADP-bound states are converted to ATP-bound states through a nucleotide exchange process (vertical solid blue lines) with rates k DT , kDTS. The ATP to ADP conversion can occur by means of either a nucleotide exchange process (dashed blue lines) with rates kTDex, kTDex,S or ATP-hydrolysis (red lines) with rates k h , khS. DOI:
http://dx.doi.org/10.7554/eLife.02218.003
Figure 2.. Effect of ATP-hydrolysis on Keffneq.
Total energy consumption (A) and effective non-equilibrium dissociation constant of the Hsp70-substrate complex (B) is plotted as a function of the hydrolysis acceleration ratio khs/kh, for the DnaK/DnaJ/substrate system with concentrations [Hsp70]tot = 40 μM and [S]tot = 4 μM (see ‘Materials and methods’ for the parameters), The approximate dissociation constant Keff,0neq is also plotted for comparison (black dashed line). The green region comprised between KD(ATP) and KD(ADP) corresponds to the range of affinities accessible at equilibrium (no hydrolysis). The red-to-yellow region corresponds to the values of the dissociation constants that are exclusively accessible to the non-equilibrium regime. The region where red fades to yellow (103 ≤ khs/kh ≤ 104) corresponds to the transition from physiological to non-physiological values of hydrolysis acceleration. DOI:
http://dx.doi.org/10.7554/eLife.02218.004
Figure 3.. Dependence of Keffneq on time-scale separation and on stoichiometric ratio.
(A) Non-equilibrium dissociation constant as a function of the hydrolysis acceleration ratio khs/kh and of the time-scale separation between the ATP- and ADP-state, expressed as the ratio between the substrate unbinding rates between the ATP- and ADP-state. (B) Non-equilibrium dissociation constant as a function of the hydrolysis acceleration ratio khs/kh and of the stoichiometric ratio between the total substrate and Hsp70 concentrations. The color codes are the same as in Figure 2, green for the region accessible in equilibrium, and red-to-yellow for the region accessible in non-equilibrium. The blue line is the non-equilibrium dissociation constant reported in Figure 2B. DOI:
http://dx.doi.org/10.7554/eLife.02218.006
Similar articles
- Efficient conversion of chemical energy into mechanical work by Hsp70 chaperones.
Assenza S, Sassi AS, Kellner R, Schuler B, De Los Rios P, Barducci A. Assenza S, et al. Elife. 2019 Dec 17;8:e48491. doi: 10.7554/eLife.48491. Elife. 2019. PMID: 31845888 Free PMC article. - Non-Equilibrium Protein Folding and Activation by ATP-Driven Chaperones.
Xu H. Xu H. Biomolecules. 2022 Jun 15;12(6):832. doi: 10.3390/biom12060832. Biomolecules. 2022. PMID: 35740957 Free PMC article. - Thermodynamic Bounds on the Ultra- and Infra-affinity of Hsp70 for Its Substrates.
Nguyen B, Hartich D, Seifert U, Rios PL. Nguyen B, et al. Biophys J. 2017 Jul 25;113(2):362-370. doi: 10.1016/j.bpj.2017.06.010. Biophys J. 2017. PMID: 28746847 Free PMC article. - Hsp70 chaperones: cellular functions and molecular mechanism.
Mayer MP, Bukau B. Mayer MP, et al. Cell Mol Life Sci. 2005 Mar;62(6):670-84. doi: 10.1007/s00018-004-4464-6. Cell Mol Life Sci. 2005. PMID: 15770419 Free PMC article. Review. - Molecular chaperones in protein folding and translocation.
Clarke AR. Clarke AR. Curr Opin Struct Biol. 1996 Feb;6(1):43-50. doi: 10.1016/s0959-440x(96)80093-5. Curr Opin Struct Biol. 1996. PMID: 8696972 Review.
Cited by
- Single-molecule evidence of Entropic Pulling by Hsp70 chaperones.
Rukes V, Rebeaud ME, Perrin LW, De Los Rios P, Cao C. Rukes V, et al. Nat Commun. 2024 Oct 8;15(1):8604. doi: 10.1038/s41467-024-52674-y. Nat Commun. 2024. PMID: 39379347 Free PMC article. - ABC transporters are billion-year-old Maxwell Demons.
Flatt S, Busiello DM, Zamuner S, De Los Rios P. Flatt S, et al. Commun Phys. 2023;6(1):205. doi: 10.1038/s42005-023-01320-y. Epub 2023 Aug 8. Commun Phys. 2023. PMID: 38665399 Free PMC article. - In-Cell Dynamics: The Next Focus of All-Atom Simulations.
Samuel Russell PP, Alaeen S, Pogorelov TV. Samuel Russell PP, et al. J Phys Chem B. 2023 Nov 23;127(46):9863-9872. doi: 10.1021/acs.jpcb.3c05166. Epub 2023 Oct 4. J Phys Chem B. 2023. PMID: 37793083 Review. - The unhappy chaperone.
Linse S, Thalberg K, Knowles TPJ. Linse S, et al. QRB Discov. 2021 Jul 8;2:e7. doi: 10.1017/qrd.2021.5. eCollection 2021. QRB Discov. 2021. PMID: 37529680 Free PMC article. - Data-driven large-scale genomic analysis reveals an intricate phylogenetic and functional landscape in J-domain proteins.
Malinverni D, Zamuner S, Rebeaud ME, Barducci A, Nillegoda NB, De Los Rios P. Malinverni D, et al. Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2218217120. doi: 10.1073/pnas.2218217120. Epub 2023 Jul 31. Proc Natl Acad Sci U S A. 2023. PMID: 37523524 Free PMC article. Review.
References
- Ge H, Qian M, Qian H. 2012. Stochastic theory of nonequilibrium steady states. Part II: applications in chemical biophysics. Physics Reports-Review Section Of Physics Letters 510:87–118
Publication types
MeSH terms
Substances
Grants and funding
The funder had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
LinkOut - more resources
Full Text Sources
Other Literature Sources