Different miRNA expression profiles between human breast cancer tumors and serum - PubMed (original) (raw)

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Different miRNA expression profiles between human breast cancer tumors and serum

Jie Zhu et al. Front Genet. 2014.

Abstract

A bunch of microRNAs (miRNAs) have been demonstrated to be aberrantly expressed in cancer tumor tissue and serum. The miRNA signatures identified from the serum samples could serve as potential noninvasive diagnostic markers for breast cancer. The role of the miRNAs in cancerigenesis is unclear. In this study, we generated the expression profiles of miRNAs from the paired breast cancer tumors, normal, tissue, and serum samples from eight patients using small RNA-sequencing. Serum samples from eight healthy individuals were used as normal controls. We identified total 174 significantly differentially expressed miRNAs between tumors and the normal tissues, and 109 miRNAs between serum from patients and serum from healthy individuals. There are only 10 common miRNAs. This suggests that only a small portion of tumor miRNAs are released into serum selectively. Interestingly, the expression change pattern of 28 miRNAs is opposite between breast cancer tumors and serum. Functional analysis shows that the differentially expressed miRNAs and their target genes form a complex interaction network affecting many biological processes and involving in many types of cancer such as prostate cancer, basal cell carcinoma, acute myeloid leukemia, and more.

Keywords: biomarker; breast cancer; miRNA; serum; tumor.

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Figures

Figure 1

Figure 1

Correlation analysis of the global miRNAs expression. (A) The scatter plot of genome-wide miRNA expression between breast cancer serum between normal serum (Pearson _r_2 = 0.98), (B). The scatter plot of genome-wide miRNA expression between breast cancer tumors between the adjacent normal tissue (Pearson _r_2 = 0.74).

Figure 2

Figure 2

Interaction network of the 10 common differentially expressed miRNAs and their target genes in breast cancer. Target genes associated with only one miRNA are indicated in dark red. Target genes associated with more than one miRNAs are indicated in pink. The 10 common miRNAs are indicated in blue.

Figure 3

Figure 3

Top 15 GO terms enriched in the up-and down-regulated target genes of the differentially miRNAs.

Figure 4

Figure 4

KEGG pathways enriched in the up-and down-regulated target genes of the differentially miRNAs by DAVID.

Figure 5

Figure 5

miR-1246 and miR-485 molecules bind to the target gene. (A) The alignment between CD59 3′ UTR and miR-1246. (B) The alignment between CD59 3′ UTR and miR-485.

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