High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors - PubMed (original) (raw)
High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors
V A Luckow et al. Virology. 1989 May.
Abstract
High levels of nonfused chloramphenicol acetyltransferase, beta-galactosidase, and beta-glucuronidase expressed under the control of new vector constructs of the polyhedrin promoter in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus were investigated by SDS-PAGE and RNA dot blot analysis of total cytoplasmic RNA. When the polyhedrin ATG start codon was converted to ATT by site-directed mutagenesis, translation initiated at downstream ATG codons resulting in high yields of nonfused foreign proteins. When a stop codon was inserted downstream from and in phase with the polyhedrin ATG codon but upstream from the ATG of a foreign gene, nonfused proteins were also produced, but at lower levels. The level of steady-state polyhedrin gene-promoted mRNA was not affected by the mutation from ATG to ATT or the insertion of in phase stop codons downstream from the polyhedrin ATG.
Similar articles
- Signals important for high-level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors.
Luckow VA, Summers MD. Luckow VA, et al. Virology. 1988 Nov;167(1):56-71. doi: 10.1016/0042-6822(88)90054-2. Virology. 1988. PMID: 3142147 - Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus.
Possee RD, Howard SC. Possee RD, et al. Nucleic Acids Res. 1987 Dec 23;15(24):10233-48. doi: 10.1093/nar/15.24.10233. Nucleic Acids Res. 1987. PMID: 3320964 Free PMC article. - Insertion of the SfMNPV polyhedrin gene into an AcMNPV polyhedrin deletion mutant during viral infection.
Gonzalez MA, Smith GE, Summers MD. Gonzalez MA, et al. Virology. 1989 May;170(1):160-75. doi: 10.1016/0042-6822(89)90363-2. Virology. 1989. PMID: 2655273 - Expression of eukaryotic genes in insect cultures.
Fraser MJ. Fraser MJ. In Vitro Cell Dev Biol. 1989 Mar;25(3 Pt 1):225-35. doi: 10.1007/BF02628459. In Vitro Cell Dev Biol. 1989. PMID: 2647707 Review. No abstract available. - Expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells: homologous viral and heterologous vertebrate genes--the baculovirus vector system.
Doerfler W. Doerfler W. Curr Top Microbiol Immunol. 1986;131:51-68. doi: 10.1007/978-3-642-71589-1_4. Curr Top Microbiol Immunol. 1986. PMID: 3545694 Review. No abstract available.
Cited by
- A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells.
Grose C, Putman Z, Esposito D. Grose C, et al. Protein Expr Purif. 2021 Oct;186:105924. doi: 10.1016/j.pep.2021.105924. Epub 2021 Jun 1. Protein Expr Purif. 2021. PMID: 34087362 Free PMC article. Review. - Novel recombinant feline interferon carrying N-glycans with reduced allergy risk produced by a transgenic silkworm system.
Minagawa S, Nakaso Y, Tomita M, Igarashi T, Miura Y, Yasuda H, Sekiguchi S. Minagawa S, et al. BMC Vet Res. 2018 Aug 31;14(1):260. doi: 10.1186/s12917-018-1584-z. BMC Vet Res. 2018. PMID: 30170576 Free PMC article. - Identification of Core Alpha 1,3-Fucosyltransferase Gene From Silkworm: An Insect Popularly Used to Express Mammalian Proteins.
Minagawa S, Sekiguchi S, Nakaso Y, Tomita M, Takahisa M, Yasuda H. Minagawa S, et al. J Insect Sci. 2015 Jul 29;15(1):110. doi: 10.1093/jisesa/iev088. Print 2015. J Insect Sci. 2015. PMID: 26223947 Free PMC article. - Immunogenicity and safety of virus-like particle of the porcine encephalomyocarditis virus in pig.
Jeoung HY, Lee WH, Jeong W, Shin BH, Choi HW, Lee HS, An DJ. Jeoung HY, et al. Virol J. 2011 Apr 15;8:170. doi: 10.1186/1743-422X-8-170. Virol J. 2011. PMID: 21492483 Free PMC article. - Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs.
Cao Y, Lu Z, Sun J, Bai X, Sun P, Bao H, Chen Y, Guo J, Li D, Liu X, Liu Z. Cao Y, et al. Vet Microbiol. 2009 May 28;137(1-2):10-7. doi: 10.1016/j.vetmic.2008.12.007. Epub 2008 Dec 11. Vet Microbiol. 2009. PMID: 19167843 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources