Brain microvessels produce 12-hydroxyeicosatetraenoic acid - PubMed (original) (raw)
Brain microvessels produce 12-hydroxyeicosatetraenoic acid
S A Moore et al. J Neurochem. 1989 Aug.
Abstract
Cerebral microvessels isolated from perfused, adult murine brain produce a compound with the chromatographic properties of a monohydroxyeicosatetraenoic acid when incubated with arachidonic acid or stimulated with calcium ionophore A23187. The formation of this arachidonic acid metabolite is not reduced in the presence of the cyclooxygenase inhibitor ibuprofen, but it is abolished by the lipoxygenase inhibitor nordihydroguaiaretic acid. Analysis by gas chromatography combined with chemical ionization and electron impact mass spectrometry of reduced and nonreduced derivatives of the metabolite, indicate that the compound is 12-hydroxyeicosatetraenoic acid. Fractions of isolated microvessels enriched with capillaries produce 2.1 times more 12-hydroxyeicosatetraenoic acid per microgram of protein than do fractions of microvessels enriched with arterioles. These studies confirm that brain microvessels can produce 12-hydroxyeicosatetraenoic acid and strongly suggest that cerebral endothelia are the primary source of microvessel-derived 12-hydroxyeicosatetraenoic acid. They further suggest that in brain injury, the liberation and accumulation of arachidonic acid in cerebral tissues may lead to the production of 12-hydroxyeicosatetraenoic acid within microvessels. The 12-hydroxyeicosatetraenoic acid formed in this way may mediate some of the blood-brain barrier and cerebrovascular dysfunction that occurs following stroke, brain trauma, or seizures.
Similar articles
- Brain microvessel 12-hydroxyeicosatetraenoic acid is the (S) enantiomer and is lipoxygenase derived.
Moore SA, Giordano MJ, Kim HY, Salem N Jr, Spector AA. Moore SA, et al. J Neurochem. 1991 Sep;57(3):922-9. doi: 10.1111/j.1471-4159.1991.tb08239.x. J Neurochem. 1991. PMID: 1907312 - Brain 12-HETE formation in different species, brain regions, and in brain microvessels.
Hambrecht GS, Adesuyi SA, Holt S, Ellis EF. Hambrecht GS, et al. Neurochem Res. 1987 Nov;12(11):1029-33. doi: 10.1007/BF00970932. Neurochem Res. 1987. PMID: 3120027 - Murine cerebral microvascular endothelium incorporate and metabolize 12-hydroxyeicosatetraenoic acid.
Moore SA, Prokuski LJ, Figard PH, Spector AA, Hart MN. Moore SA, et al. J Cell Physiol. 1988 Oct;137(1):75-85. doi: 10.1002/jcp.1041370109. J Cell Physiol. 1988. PMID: 3170659 - Arachidonic acid metabolism of cytosolic fractions of Lewis lung carcinoma cells.
Marnett LJ, Leithauser MT, Richards KM, Blair I, Honn KV, Yamamoto S, Yoshimoto T. Marnett LJ, et al. Adv Prostaglandin Thromboxane Leukot Res. 1991;21B:895-900. Adv Prostaglandin Thromboxane Leukot Res. 1991. PMID: 1825434 Review. No abstract available. - 12(R)- and 12(S)-hydroxyeicosatetraenoic acids: chemistry, biology, and pharmacology.
Fretland DJ, Djuric SW. Fretland DJ, et al. Prostaglandins Leukot Essent Fatty Acids. 1989 Dec;38(4):215-28. doi: 10.1016/0952-3278(89)90125-7. Prostaglandins Leukot Essent Fatty Acids. 1989. PMID: 2517456 Review. No abstract available.
Cited by
- Endothelial 12(S)-HETE vasorelaxation is mediated by thromboxane receptor inhibition in mouse mesenteric arteries.
Siangjong L, Gauthier KM, Pfister SL, Smyth EM, Campbell WB. Siangjong L, et al. Am J Physiol Heart Circ Physiol. 2013 Feb 1;304(3):H382-92. doi: 10.1152/ajpheart.00690.2012. Epub 2012 Nov 30. Am J Physiol Heart Circ Physiol. 2013. PMID: 23203967 Free PMC article. - 12-Hydroxyeicosatetraenoic acid is the only enzymatically produced HETE increased under brain ischemia.
Golovko MY, Seeger DR, Schofield B, Besch D, Kotha P, Mansouripour A, Solaymani-Mohammadi S, Golovko SA. Golovko MY, et al. Prostaglandins Leukot Essent Fatty Acids. 2024 Mar;202:102631. doi: 10.1016/j.plefa.2024.102631. Epub 2024 Jul 19. Prostaglandins Leukot Essent Fatty Acids. 2024. PMID: 39059107 - Mechanisms of blood-brain barrier breakdown.
Greenwood J. Greenwood J. Neuroradiology. 1991;33(2):95-100. doi: 10.1007/BF00588242. Neuroradiology. 1991. PMID: 2046916 Review. - Dietary manipulation with high marine fish oil intake of fatty acid composition and arachidonic acid metabolism in rat cerebral microvessels.
Kálmán J, Gecse A, Farkas T, Joó F, Telegdy G, Lajtha A. Kálmán J, et al. Neurochem Res. 1992 Feb;17(2):167-72. doi: 10.1007/BF00966795. Neurochem Res. 1992. PMID: 1531701
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources