Biased immunoglobulin variable region gene expression by Ly-1 B cells due to clonal selection - PubMed (original) (raw)
Biased immunoglobulin variable region gene expression by Ly-1 B cells due to clonal selection
C A Pennell et al. Eur J Immunol. 1989 Jul.
Abstract
Most, if not all, autoantibodies specific for bromelain-treated mouse erythrocytes recognize the common membrane phospholipid, phosphatidyl choline (PtC). Anti-PtC antibodies are produced by 5%-15% of CD5+ Ly-1 B cells of normal unimmunized mice, but not by detectable numbers of conventional CD5- B cells. At 1 week of age PtC-specific B cells are undetectable but then increase dramatically over the next 3 to 4 weeks to reach adult numbers. We report here that PtC-specific Ly-1 B cells in B10.H-2aH-4bp/Wts mice predominantly express either of two heavy and kappa chain variable (V) region gene combinations. In addition, the sequence and length of DH genes are conserved among cells expressing the same V gene combination, and the V kappa-J kappa junctions of one group involve unusual splice sites. Preferential V gene rearrangement models are insufficient to explain the DH and V kappa-J kappa junctional sequences or the delayed appearance of this specificity, and so they cannot solely account for the high frequency of PtC-specific cells. These characteristics are more consistent with antigen selection. We therefore attribute the frequent use of the two V region gene combinations to selection for cells that express them and conclude that the expressed V gene repertoire of Ly-1 B cells in adult mice is influenced by antigen selection. Apparently, there is no selection for mutant anti-PtC antibodies of higher affinity during the formation of the Ly-1 B repertoire because the V region genes expressed by PtC-specific cells are unmutated. Our findings are consistent with an important, germ line-encoded function for the immunoglobulin products of these gene combinations.
Similar articles
- High frequency expression of S107 VH genes by peritoneal B cells of B10.H-2aH-4bP/WTS mice.
Pennell CA, Maynard E, Arnold LW, Haughton G, Clarke SH. Pennell CA, et al. J Immunol. 1990 Sep 1;145(5):1592-7. J Immunol. 1990. PMID: 2117034 - A single VH gene is utilized predominantly in anti-BrMRBC hybridomas derived from purified Ly-1 B cells. Definition of the VH11 family.
Hardy RR, Carmack CE, Shinton SA, Riblet RJ, Hayakawa K. Hardy RR, et al. J Immunol. 1989 May 15;142(10):3643-51. J Immunol. 1989. PMID: 2497178 - Neonatal and adult primary B cells use the same germ-line VH and V kappa genes in their (T,G)-A-L-specific repertoire.
Borriero L, Giorgetti C, Smith G, Landry D, Selsing E, Zhukovsky E, Press JL. Borriero L, et al. J Immunol. 1990 Jan 15;144(2):583-92. J Immunol. 1990. PMID: 2129539 - The expressed heavy chain V gene repertoire of circulating B cells in normal adults.
Stollar BD. Stollar BD. Ann N Y Acad Sci. 1995 Sep 29;764:265-74. doi: 10.1111/j.1749-6632.1995.tb55836.x. Ann N Y Acad Sci. 1995. PMID: 7486534 Review. No abstract available. - Immunoglobulin V genes expression and mRNA sequencing in rheumatoid arthritis.
Haynes GA, Maini RN, Malcolm AD. Haynes GA, et al. Ann Rheum Dis. 1990 Jun;49 Suppl 1:460-8. Ann Rheum Dis. 1990. PMID: 2197997 Review.
Cited by
- New insights into heterogeneity of peritoneal B-1a cells.
Wang H, Lin JX, Li P, Skinner J, Leonard WJ, Morse HC 3rd. Wang H, et al. Ann N Y Acad Sci. 2015 Dec;1362(1):68-76. doi: 10.1111/nyas.12791. Epub 2015 May 18. Ann N Y Acad Sci. 2015. PMID: 25988856 Free PMC article. Review. - Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+ CD27+ CD43+ CD70-.
Griffin DO, Holodick NE, Rothstein TL. Griffin DO, et al. J Exp Med. 2011 Jan 17;208(1):67-80. doi: 10.1084/jem.20101499. Epub 2011 Jan 10. J Exp Med. 2011. PMID: 21220451 Free PMC article. - Adult BM generates CD5+ B1 cells containing abundant N-region additions.
Holodick NE, Repetny K, Zhong X, Rothstein TL. Holodick NE, et al. Eur J Immunol. 2009 Sep;39(9):2383-94. doi: 10.1002/eji.200838920. Eur J Immunol. 2009. PMID: 19714574 Free PMC article. - Facultative role of germinal centers and T cells in the somatic diversification of IgVH genes.
Miller C, Stedra J, Kelsoe G, Cerny J. Miller C, et al. J Exp Med. 1995 Apr 1;181(4):1319-31. doi: 10.1084/jem.181.4.1319. J Exp Med. 1995. PMID: 7535332 Free PMC article. - Rapid cloning of any rearranged mouse immunoglobulin variable genes.
Dattamajumdar AK, Jacobson DP, Hood LE, Osman GE. Dattamajumdar AK, et al. Immunogenetics. 1996;43(3):141-51. doi: 10.1007/BF00176675. Immunogenetics. 1996. PMID: 8550098
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources