Three-dimensional solution structure of a single zinc finger DNA-binding domain - PubMed (original) (raw)
. 1989 Aug 11;245(4918):635-7.
doi: 10.1126/science.2503871.
Affiliations
- PMID: 2503871
- DOI: 10.1126/science.2503871
Three-dimensional solution structure of a single zinc finger DNA-binding domain
M S Lee et al. Science. 1989.
Abstract
The three-dimensional solution structure of a zinc finger nucleic acid binding motif has been determined by nuclear magnetic resonance (NMR) spectroscopy. Spectra of a synthetic peptide corresponding to a single zinc finger from the Xenopus protein Xfin yielded distance and dihedral angle constraints that were used to generate structures from distance geometry and restrained molecular dynamics calculations. The zinc finger is an independently folded domain with a compact globular structure in which the zinc atom is bound by two cysteine and two histidine ligands. The polypeptide backbone fold consists of a well-defined helix, starting as alpha and ending as 3(10) helix, packed against two beta strands that are arranged in a hairpin structure. A high density of basic and polar amino acid side chains on the exposed face of the helix are probably involved in DNA binding.
Similar articles
- High-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution.
Omichinski JG, Clore GM, Appella E, Sakaguchi K, Gronenborn AM. Omichinski JG, et al. Biochemistry. 1990 Oct 9;29(40):9324-34. doi: 10.1021/bi00492a004. Biochemistry. 1990. PMID: 2248949 - Complete assignment of the 1H NMR spectrum of a synthetic zinc finger from Xfin. Sequential resonance assignments and secondary structure.
Lee MS, Cavanagh J, Wright PE. Lee MS, et al. FEBS Lett. 1989 Aug 28;254(1-2):159-64. doi: 10.1016/0014-5793(89)81030-0. FEBS Lett. 1989. PMID: 2506074 - Structure of a histidine-X4-histidine zinc finger domain: insights into ADR1-UAS1 protein-DNA recognition.
Bernstein BE, Hoffman RC, Horvath S, Herriott JR, Klevit RE. Bernstein BE, et al. Biochemistry. 1994 Apr 19;33(15):4460-70. doi: 10.1021/bi00181a005. Biochemistry. 1994. PMID: 8161501 - Zinc fingers and molecular recognition. Structure and nucleic acid binding studies of an HIV zinc finger-like domain.
South TL, Kim B, Hare DR, Summers MF. South TL, et al. Biochem Pharmacol. 1990 Jul 1;40(1):123-9. doi: 10.1016/0006-2952(90)90187-p. Biochem Pharmacol. 1990. PMID: 2115335 Review. No abstract available. - Zinc finger motif for single-stranded nucleic acids? Investigations by nuclear magnetic resonance.
Summers MF. Summers MF. J Cell Biochem. 1991 Jan;45(1):41-8. doi: 10.1002/jcb.240450110. J Cell Biochem. 1991. PMID: 2005183 Review.
Cited by
- Formerly degenerate seventh zinc finger domain from transcription factor ZNF711 rehabilitated by experimental NMR structure.
Rua AJ, Alexandrescu AT. Rua AJ, et al. Protein Sci. 2024 Sep;33(9):e5149. doi: 10.1002/pro.5149. Protein Sci. 2024. PMID: 39180464 - Comprehensive pan-cancer analysis of ZNF337 as a potential diagnostic, immunological, and prognostic biomarker.
Zhang D, Liang P, Xia B, Wu J, Hu X. Zhang D, et al. BMC Cancer. 2024 Aug 9;24(1):987. doi: 10.1186/s12885-024-12703-x. BMC Cancer. 2024. PMID: 39123194 Free PMC article. - Structural insights into the cooperative nucleosome recognition and chromatin opening by FOXA1 and GATA4.
Zhou BR, Feng H, Huang F, Zhu I, Portillo-Ledesma S, Shi D, Zaret KS, Schlick T, Landsman D, Wang Q, Bai Y. Zhou BR, et al. Mol Cell. 2024 Aug 22;84(16):3061-3079.e10. doi: 10.1016/j.molcel.2024.07.016. Epub 2024 Aug 8. Mol Cell. 2024. PMID: 39121853 - Molecular Evolution of RAMOSA1 (RA1) in Land Plants.
Bellino C, Herrera FE, Rodrigues D, Garay AS, Huck SV, Reinheimer R. Bellino C, et al. Biomolecules. 2024 May 3;14(5):550. doi: 10.3390/biom14050550. Biomolecules. 2024. PMID: 38785957 Free PMC article. - System-Specific Parameter Optimization for Nonpolarizable and Polarizable Force Fields.
Hu X, Amin KS, Schneider M, Lim C, Salahub D, Baldauf C. Hu X, et al. J Chem Theory Comput. 2024 Feb 13;20(3):1448-1464. doi: 10.1021/acs.jctc.3c01141. Epub 2024 Jan 27. J Chem Theory Comput. 2024. PMID: 38279917 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources