Insertion and amplification of foreign genes in the Lactococcus lactis subsp. lactis chromosome - PubMed (original) (raw)
Insertion and amplification of foreign genes in the Lactococcus lactis subsp. lactis chromosome
M C Chopin et al. Appl Environ Microbiol. 1989 Jul.
Abstract
The plasmid pE194 is unable to replicate in Lactococcus lactis subsp. lactis (formerly Streptococcus lactis). When linked to resident bacteriophage sequences, pE194 was able to integrate into the L. lactis subsp. lactis chromosome either by Campbell-like recombination or by double crossing over with deletion. Integration occurred into the DNA of the prophage and prevented its multiplication. When a selective pressure was applied to an integrant in which pE194 was flanked by two direct repeats of prophage fragment, amplification of pE194 and the prophage fragment was observed. The pE194 copy number was assessed at six to nine, and amplification was stable upon growth under nonselective conditions.
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References
- Proc Natl Acad Sci U S A. 1979 Oct;76(10):5259-63 - PubMed
- Appl Environ Microbiol. 1986 Aug;52(2):394-5 - PubMed
- EMBO J. 1984 Dec 1;3(12):2879-84 - PubMed
- Appl Environ Microbiol. 1982 Feb;43(2):349-56 - PubMed
- Gene. 1985;40(1):47-55 - PubMed
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