Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse - PubMed (original) (raw)

Comparative Study

Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse

M Strathmann et al. Proc Natl Acad Sci U S A. 1989 Oct.

Abstract

Biochemical analysis has revealed a number of guanine nucleotide-binding regulatory proteins (G proteins) that mediate signal transduction in mammalian systems. Characterization of their cDNAs uncovered a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of diversity of the G protein family, we used the polymerase chain reaction to detect additional gene products in mouse brain and spermatid RNA that share these conserved regions. Sequences corresponding to six of the eight known G protein alpha subunits were obtained. In addition, we found sequences corresponding to five newly discovered alpha subunits. Our results suggest that the complexity of the G protein family is much greater than previously suspected.

PubMed Disclaimer

Similar articles

• Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go.
  Strathmann M, Wilkie TM, Simon MI. Strathmann M, et al. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6477-81. doi: 10.1073/pnas.87.17.6477. Proc Natl Acad Sci U S A. 1990. PMID: 1697681 Free PMC article.
• G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.
  Strathmann M, Simon MI. Strathmann M, et al. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9113-7. doi: 10.1073/pnas.87.23.9113. Proc Natl Acad Sci U S A. 1990. PMID: 2123549 Free PMC article.
• Dictyostelium transiently expresses eight distinct G-protein alpha-subunits during its developmental program.
  Wu LJ, Devreotes PN. Wu LJ, et al. Biochem Biophys Res Commun. 1991 Sep 30;179(3):1141-7. doi: 10.1016/0006-291x(91)91690-e. Biochem Biophys Res Commun. 1991. PMID: 1930158
• Evolution of the mammalian G protein alpha subunit multigene family.
  Wilkie TM, Gilbert DJ, Olsen AS, Chen XN, Amatruda TT, Korenberg JR, Trask BJ, de Jong P, Reed RR, Simon MI, et al. Wilkie TM, et al. Nat Genet. 1992 May;1(2):85-91. doi: 10.1038/ng0592-85. Nat Genet. 1992. PMID: 1302014 Review.
• Structures of the genes coding for G-protein alpha subunits from mammalian and yeast cells.
  Kaziro Y, Itoh H, Kozasa T, Toyama R, Tsukamoto T, Matsuoka M, Nakafuku M, Obara T, Takagi T, Hernandez R. Kaziro Y, et al. Cold Spring Harb Symp Quant Biol. 1988;53 Pt 1:209-20. doi: 10.1101/sqb.1988.053.01.027. Cold Spring Harb Symp Quant Biol. 1988. PMID: 3151168 Review. No abstract available.

Cited by

• Cloning and expression of a Ca(2+)-inhibitable adenylyl cyclase from NCB-20 cells.
  Yoshimura M, Cooper DM. Yoshimura M, et al. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6716-20. doi: 10.1073/pnas.89.15.6716. Proc Natl Acad Sci U S A. 1992. PMID: 1379717 Free PMC article.
• Expansion of signal transduction by G proteins. The second 15 years or so: from 3 to 16 alpha subunits plus betagamma dimers.
  Birnbaumer L. Birnbaumer L. Biochim Biophys Acta. 2007 Apr;1768(4):772-93. doi: 10.1016/j.bbamem.2006.12.002. Epub 2006 Dec 15. Biochim Biophys Acta. 2007. PMID: 17258171 Free PMC article.
• Deletion of steroid 5 alpha-reductase 2 gene in male pseudohermaphroditism.
  Andersson S, Berman DM, Jenkins EP, Russell DW. Andersson S, et al. Nature. 1991 Nov 14;354(6349):159-61. doi: 10.1038/354159a0. Nature. 1991. PMID: 1944596 Free PMC article.
• AGS3 and Gαi3 Are Concomitantly Upregulated as Part of the Spindle Orientation Complex during Differentiation of Human Neural Progenitor Cells.
  Yip JLK, Lee MMK, Leung CCY, Tse MK, Cheung AST, Wong YH. Yip JLK, et al. Molecules. 2020 Nov 6;25(21):5169. doi: 10.3390/molecules25215169. Molecules. 2020. PMID: 33172018 Free PMC article.
• Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go.
  Strathmann M, Wilkie TM, Simon MI. Strathmann M, et al. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6477-81. doi: 10.1073/pnas.87.17.6477. Proc Natl Acad Sci U S A. 1990. PMID: 1697681 Free PMC article.

References

1. 1. Biochemistry. 1988 Jul 12;27(14):4957-65 - PubMed
2. 1. J Biol Chem. 1988 Aug 25;263(24):12070-6 - PubMed
3. 1. Protein Eng. 1986 Oct-Nov;1(1):47-54 - PubMed
4. 1. Cell. 1989 Apr 21;57(2):265-75 - PubMed
5. 1. Science. 1989 May 19;244(4906):790-5 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • Mouse Genome Informatics (MGI)