Role of sigma H in expression of the fumarase gene (citG) in vegetative cells of Bacillus subtilis 168 - PubMed (original) (raw)
Role of sigma H in expression of the fumarase gene (citG) in vegetative cells of Bacillus subtilis 168
V A Price et al. J Bacteriol. 1989 Nov.
Abstract
The fumarase gene (citG) of Bacillus subtilis is transcribed from two promoter regions, citGp1 and citGp2 (P1 and P2); the P2 promoter is used by the E sigma H form of RNA polymerase. In order to study the role of P1 and P2 in citG expression, the promoter region and various deletion derivatives that effectively separate P1 and P2 were fused to the Escherichia coli beta-galactosidase gene (lacZ) and introduced into the chromosome in single copy at the amyE locus. P1 functioned to provide a relatively low and stable basal level of fumarase activity throughout growth. In contrast, P2 activity was found to vary over at least a 50-fold range and was responsible for regulating fumarase activity during growth and sporulation in a rich medium and in response to changes in carbon source. To further investigate the role of sigma H in fumarase regulation, citGp2-lacZ fusions were introduced into a strain in which the expression of the chromosomal spoOH gene was under the control of the isopropylthiogalactopyranoside-inducible spac promoter. Induction of pspac did not lead to P2 induction, suggesting that citG expression is not regulated at the level of spoOH transcription.
Similar articles
- Sigma H-directed transcription of citG in Bacillus subtilis.
Tatti KM, Carter HL 3rd, Moir A, Moran CP Jr. Tatti KM, et al. J Bacteriol. 1989 Nov;171(11):5928-32. doi: 10.1128/jb.171.11.5928-5932.1989. J Bacteriol. 1989. PMID: 2509422 Free PMC article. - The regulation of the fumarase (citG) gene of Bacillus subtilis 168.
Feavers IM, Price V, Moir A. Feavers IM, et al. Mol Gen Genet. 1988 Mar;211(3):465-71. doi: 10.1007/BF00425702. Mol Gen Genet. 1988. PMID: 3130545 - Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168.
Miles JS, Guest JR. Miles JS, et al. Nucleic Acids Res. 1985 Jan 11;13(1):131-40. doi: 10.1093/nar/13.1.131. Nucleic Acids Res. 1985. PMID: 3923430 Free PMC article. - Multiple procaryotic ribonucleic acid polymerase sigma factors.
Doi RH, Wang LF. Doi RH, et al. Microbiol Rev. 1986 Sep;50(3):227-43. doi: 10.1128/mr.50.3.227-243.1986. Microbiol Rev. 1986. PMID: 3095618 Free PMC article. Review. No abstract available.
Cited by
- Analysis of Spo0M function in Bacillus subtilis.
Vega-Cabrera LA, Guerrero A, Rodríguez-Mejía JL, Tabche ML, Wood CD, Gutiérrez-Rios RM, Merino E, Pardo-López L. Vega-Cabrera LA, et al. PLoS One. 2017 Feb 24;12(2):e0172737. doi: 10.1371/journal.pone.0172737. eCollection 2017. PLoS One. 2017. PMID: 28234965 Free PMC article. - Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis.
Britton RA, Eichenberger P, Gonzalez-Pastor JE, Fawcett P, Monson R, Losick R, Grossman AD. Britton RA, et al. J Bacteriol. 2002 Sep;184(17):4881-90. doi: 10.1128/JB.184.17.4881-4890.2002. J Bacteriol. 2002. PMID: 12169614 Free PMC article. - Characterization of the parB-like yyaA gene of Bacillus subtilis.
Sievers J, Raether B, Perego M, Errington J. Sievers J, et al. J Bacteriol. 2002 Feb;184(4):1102-11. doi: 10.1128/jb.184.4.1102-1111.2002. J Bacteriol. 2002. PMID: 11807071 Free PMC article. - Developmental gene expression in Bacillus subtilis crsA47 mutants reveals glucose-activated control of the gene for the minor sigma factor sigma(H).
Dixon LG, Seredick S, Richer M, Spiegelman GB. Dixon LG, et al. J Bacteriol. 2001 Aug;183(16):4814-22. doi: 10.1128/JB.183.16.4814-4822.2001. J Bacteriol. 2001. PMID: 11466285 Free PMC article. - The yvyD gene of Bacillus subtilis is under dual control of sigmaB and sigmaH.
Drzewiecki K, Eymann C, Mittenhuber G, Hecker M. Drzewiecki K, et al. J Bacteriol. 1998 Dec;180(24):6674-80. doi: 10.1128/JB.180.24.6674-6680.1998. J Bacteriol. 1998. PMID: 9852014 Free PMC article.
References
- J Bacteriol. 1986 Nov;168(2):870-7 - PubMed
- J Bacteriol. 1967 Jun;93(6):1777-87 - PubMed
- J Bacteriol. 1961 May;81(5):741-6 - PubMed
- J Bacteriol. 1987 Aug;169(8):3464-9 - PubMed
- J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases