Esterase electrophoresis: a molecular tool for studying the epidemiology of Branhamella catarrhalis nosocomial infection - PubMed (original) (raw)

Comparative Study

Esterase electrophoresis: a molecular tool for studying the epidemiology of Branhamella catarrhalis nosocomial infection

B Picard et al. Epidemiol Infect. 1989 Dec.

Abstract

A new epidemiologic typing method based on electrophoresis of esterases had been developed for differentiating between clinical isolates of Branhamella catarrhalis. Twenty-two epidemiologically significant strains obtained from three Chest Units, a Paediatric Intensive Care Unit and a Paediatric Unit were compared with 54 randomly selected strains and 4 reference strains, including the species type strain, ATCC 25238. Thirty-four distinct zymotypes were characterized by polyacrylamide-agarose gel electrophoresis of the 80 strains. One infrequent zymotype was found in 2 neonates and another in 2 adults with nosocomial bronchopulmonary infections, suggesting the nosocomial spread of 2 outbreak strains of B. catarrhalis. A more frequent zymotype was isolated from 3 neonates with nosocomial bronchopulmonary infection and from 2 children with nosocomial rhinopharyngitis. The remaining 12 epidemiologically significant strains were of varied zymotypes. This work demonstrates that esterase electrophoresis is a suitable, readily reproducible, stable typing system applicable to the wide range of strains found in B. catarrhalis nosocomial infections.

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References

1. 1. Bull Soc Chim Biol (Paris). 1966;48(8):969-82 - PubMed
2. 1. Virology. 1955 Jul;1(2):190-206 - PubMed
3. 1. Am J Med. 1981 Apr;70(4):899-905 - PubMed
4. 1. Presse Med. 1984 Apr 21;13(17):1079-81 - PubMed
5. 1. J Hosp Infect. 1984 Sep;5(3):335-7 - PubMed

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