SATB2 enhances migration and invasion in osteosarcoma by regulating genes involved in cytoskeletal organization - PubMed (original) (raw)

. 2015 Jul;34(27):3582-92.

doi: 10.1038/onc.2014.289. Epub 2014 Sep 15.

Affiliations

SATB2 enhances migration and invasion in osteosarcoma by regulating genes involved in cytoskeletal organization

B K A Seong et al. Oncogene. 2015 Jul.

Abstract

Osteosarcoma (OS) is the most common malignant bone tumor and the majority of recurrences are due to metastasis. However, the molecular mechanisms that regulate OS metastatic spread are largely unknown. In this study, we report that special AT-rich-binding protein 2 (SATB2) is highly expressed in OS cells and tumors. Short hairpin RNA-mediated knockdown of SATB2 (sh-SATB2) decreases migration and invasion of OS cells without affecting proliferation or viability. Microarray analysis identified genes that were differentially regulated by SATB2 including the actin-binding protein Epithelial Protein Lost In Neoplasm (EPLIN), which was upregulated in sh-SATB2 cells. Silencing EPLIN rescues the decreased invasion observed in sh-SATB2 cells. Pathway analyses of SATB2-regulated genes revealed enrichment of those involved in cytoskeleton dynamics, and increased stress fiber formation was detected in cells with SATB2 knockdown. Furthermore, sh-SATB2 cells exhibit increased RhoA, decreased Rac1 and increased phosphorylation of focal adhesion kinase (FAK) and paxillin. These findings identify SATB2 as a novel regulator of OS invasion, in part via effects on EPLIN and the cytoskeleton.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Cell Biol. 2003 Feb 3;160(3):399-407 - PubMed
    1. Cancer Res. 2012 Apr 1;72(7):1865-77 - PubMed
    1. Neuron. 2008 Feb 7;57(3):378-92 - PubMed
    1. PLoS One. 2010 Nov 15;5(11):e13984 - PubMed
    1. Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19879-84 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources