Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis - PubMed (original) (raw)
Review
Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis
Edward C Stack et al. Methods. 2014 Nov.
Free article
Abstract
Tissue sections offer the opportunity to understand a patient's condition, to make better prognostic evaluations and to select optimum treatments, as evidenced by the place pathology holds today in clinical practice. Yet, there is a wealth of information locked up in a tissue section that is only partially accessed, due mainly to the limitations of tools and methods. Often tissues are assessed primarily based on visual analysis of one or two proteins, or 2-3 DNA or RNA molecules. Even while analysis is still based on visual perception, image analysis is starting to address the variability of human perception. This is in contrast to measuring characteristics that are substantially out of reach of human perception, such as parameters revealed through co-expression, spatial relationships, heterogeneity, and low abundance molecules. What is not routinely accessed is the information revealed through simultaneous detection of multiple markers, the spatial relationships among cells and tissue in disease, and the heterogeneity now understood to be critical to developing effective therapeutic strategies. Our purpose here is to review and assess methods for multiplexed, quantitative, image analysis based approaches, using new multicolor immunohistochemistry methods, automated multispectral slide imaging, and advanced trainable pattern recognition software. A key aspect of our approach is presenting imagery in a workflow that engages the pathologist to utilize the strengths of human perception and judgment, while significantly expanding the range of metrics collectable from tissue sections and also provide a level of consistency and precision needed to support the complexities of personalized medicine.
Keywords: Biomarkers; Cancer; Multiplexed; Multispectral; Pathology; Quantitative; TSA.
Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.
Similar articles
- Digital pathology and image analysis in tissue biomarker research.
Hamilton PW, Bankhead P, Wang Y, Hutchinson R, Kieran D, McArt DG, James J, Salto-Tellez M. Hamilton PW, et al. Methods. 2014 Nov;70(1):59-73. doi: 10.1016/j.ymeth.2014.06.015. Epub 2014 Jul 15. Methods. 2014. PMID: 25034370 Review. - Multispectral imaging: a review of its technical aspects and applications in anatomic pathology.
Mansfield JR. Mansfield JR. Vet Pathol. 2014 Jan;51(1):185-210. doi: 10.1177/0300985813506918. Epub 2013 Oct 15. Vet Pathol. 2014. PMID: 24129898 Review. - Applications of pathology-assisted image analysis of immunohistochemistry-based biomarkers in oncology.
Shinde V, Burke KE, Chakravarty A, Fleming M, McDonald AA, Berger A, Ecsedy J, Blakemore SJ, Tirrell SM, Bowman D. Shinde V, et al. Vet Pathol. 2014 Jan;51(1):292-303. doi: 10.1177/0300985813511124. Epub 2013 Nov 14. Vet Pathol. 2014. PMID: 24232190 - Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging.
Bauman TM, Ricke EA, Drew SA, Huang W, Ricke WA. Bauman TM, et al. J Vis Exp. 2016 Apr 8;(110):53837. doi: 10.3791/53837. J Vis Exp. 2016. PMID: 27167094 Free PMC article. - Semi-automated imaging system to quantitate estrogen and progesterone receptor immunoreactivity in human breast cancer.
Sharangpani GM, Joshi AS, Porter K, Deshpande AS, Keyhani S, Naik GA, Gholap AS, Barsky SH. Sharangpani GM, et al. J Microsc. 2007 Jun;226(Pt 3):244-55. doi: 10.1111/j.1365-2818.2007.01772.x. J Microsc. 2007. PMID: 17535263
Cited by
- Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain.
Kang J, Schroeder ME, Lee Y, Kapoor C, Yu E, Tarr TB, Titterton K, Zeng M, Park D, Niederst E, Wei D, Feng G, Boyden ES. Kang J, et al. Nat Commun. 2024 Nov 9;15(1):9722. doi: 10.1038/s41467-024-53729-w. Nat Commun. 2024. PMID: 39521775 Free PMC article. - Distinguishing of Histopathological Staging Features of H-E Stained Human cSCC by Microscopical Multispectral Imaging.
Wu R, Yang J, Chen Q, Yang C, Ge Q, Rui D, Xiang H, Zhang D, Wang C, Zhao X. Wu R, et al. Biosensors (Basel). 2024 Sep 29;14(10):467. doi: 10.3390/bios14100467. Biosensors (Basel). 2024. PMID: 39451680 Free PMC article. - Multiplexed Immunophenotyping of Lymphoma Tissue Samples.
Schniederjohann C, Bruch PM, Dietrich S, Neumann F. Schniederjohann C, et al. Methods Mol Biol. 2025;2865:375-393. doi: 10.1007/978-1-0716-4188-0_16. Methods Mol Biol. 2025. PMID: 39424733 - NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals.
Mougios N, Cotroneo ER, Imse N, Setzke J, Rizzoli SO, Simeth NA, Tsukanov R, Opazo F. Mougios N, et al. Nat Commun. 2024 Oct 10;15(1):8771. doi: 10.1038/s41467-024-53030-w. Nat Commun. 2024. PMID: 39384781 Free PMC article. - Higher Nodal expression is often associated with poorer survival in patients diagnosed with melanoma and treated with anti-PD1 therapy.
Gascard PD, Wang X, Nosrati M, Kim KB, Kashani-Sabet M, Tlsty TD, Leong SP, Hendrix MJC. Gascard PD, et al. Pathol Oncol Res. 2024 Sep 23;30:1611889. doi: 10.3389/pore.2024.1611889. eCollection 2024. Pathol Oncol Res. 2024. PMID: 39376672 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials