Steroid biochemistry - PubMed (original) (raw)
Review
doi: 10.1159/000363612. Epub 2014 Sep 9.
Affiliations
- PMID: 25247643
- DOI: 10.1159/000363612
Review
Steroid biochemistry
Clemens Kamrath et al. Endocr Dev. 2014.
Abstract
Accurate analysis of steroid hormones represents an essential part in the evaluation of a patient with disorders or differences in sex development. Analytical methods based on mass spectrometry (MS) have become the state-of-the-art methodology allowing for the most specific qualitative and quantitative determination of steroid hormones and their metabolites. Liquid chromatography linked with tandem MS (LC-MS/MS) allows for rapid as well as highly specific and sensitive targeted steroid hormone analysis of multiple analytes from a single sample. Urinary steroid profile analysis by gas chromatography (GC)-MS is a non-invasive diagnostic approach and provides qualitative and quantitative data on the global excretion of steroid hormone metabolites. GC-MS remains the most powerful discovery tool for defining inborn errors of steroidogenesis, whereas LC-MS/MS represents a highly sensitive and specific method for targeted steroid hormone analysis.
© 2014 S. Karger AG, Basel.
Similar articles
- Measurement of a Comprehensive Sex Steroid Profile in Rodent Serum by High-Sensitive Gas Chromatography-Tandem Mass Spectrometry.
Nilsson ME, Vandenput L, Tivesten Å, Norlén AK, Lagerquist MK, Windahl SH, Börjesson AE, Farman HH, Poutanen M, Benrick A, Maliqueo M, Stener-Victorin E, Ryberg H, Ohlsson C. Nilsson ME, et al. Endocrinology. 2015 Jul;156(7):2492-502. doi: 10.1210/en.2014-1890. Epub 2015 Apr 9. Endocrinology. 2015. PMID: 25856427 - Gas chromatography/mass spectrometry (GC/MS) remains a pre-eminent discovery tool in clinical steroid investigations even in the era of fast liquid chromatography tandem mass spectrometry (LC/MS/MS).
Krone N, Hughes BA, Lavery GG, Stewart PM, Arlt W, Shackleton CH. Krone N, et al. J Steroid Biochem Mol Biol. 2010 Aug;121(3-5):496-504. doi: 10.1016/j.jsbmb.2010.04.010. Epub 2010 Apr 22. J Steroid Biochem Mol Biol. 2010. PMID: 20417277 Free PMC article. Review. - Gas chromatography-mass spectrometry.
Honour JW. Honour JW. Methods Mol Biol. 2006;324:53-74. doi: 10.1385/1-59259-986-9:53. Methods Mol Biol. 2006. PMID: 16761371 Review. - The art of measuring steroids: Principles and practice of current hormonal steroid analysis.
Wudy SA, Schuler G, Sánchez-Guijo A, Hartmann MF. Wudy SA, et al. J Steroid Biochem Mol Biol. 2018 May;179:88-103. doi: 10.1016/j.jsbmb.2017.09.003. Epub 2017 Sep 28. J Steroid Biochem Mol Biol. 2018. PMID: 28962971 Review. - Androgen glucuronides analysis by liquid chromatography tandem-mass spectrometry: could it raise new perspectives in the diagnostic field of hormone-dependent malignancies?
Kalogera E, Pistos C, Provatopoulou X, Athanaselis S, Spiliopoulou C, Gounaris A. Kalogera E, et al. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec 1;940:24-34. doi: 10.1016/j.jchromb.2013.09.022. Epub 2013 Sep 27. J Chromatogr B Analyt Technol Biomed Life Sci. 2013. PMID: 24140653 Review.
Cited by
- 46,XX DSD: Developmental, Clinical and Genetic Aspects.
Alkhzouz C, Bucerzan S, Miclaus M, Mirea AM, Miclea D. Alkhzouz C, et al. Diagnostics (Basel). 2021 Jul 30;11(8):1379. doi: 10.3390/diagnostics11081379. Diagnostics (Basel). 2021. PMID: 34441313 Free PMC article. Review. - Accuracy of Urinary Etiocholanolone/Androsterone Ratio as Alternative to Serum Testosterone/Dihydrotestosterone Ratio for Diagnosis of 5 Alpha-reductase Type 2 Deficiency Patients and Carriers in Indonesia.
Marzuki NS, Idris FP, Kartapradja H, Renata S, Harahap A, Batubara JRL. Marzuki NS, et al. Int J Endocrinol Metab. 2021 Apr 18;19(2):e109510. doi: 10.5812/ijem.109510. eCollection 2021 Apr. Int J Endocrinol Metab. 2021. PMID: 34149847 Free PMC article. - Characterising SRD5A2 Gene Variants in 37 Indonesian Patients with 5-Alpha-Reductase Type 2 Deficiency.
Marzuki NS, Idris FP, Kartapradja HD, Harahap AR, Batubara JRL. Marzuki NS, et al. Int J Endocrinol. 2019 Dec 1;2019:7676341. doi: 10.1155/2019/7676341. eCollection 2019. Int J Endocrinol. 2019. PMID: 31885560 Free PMC article. - Steroidomics for the Prevention, Assessment, and Management of Cancers: A Systematic Review and Functional Analysis.
Anh NH, Long NP, Kim SJ, Min JE, Yoon SJ, Kim HM, Yang E, Hwang ES, Park JH, Hong SS, Kwon SW. Anh NH, et al. Metabolites. 2019 Sep 21;9(10):199. doi: 10.3390/metabo9100199. Metabolites. 2019. PMID: 31546652 Free PMC article. Review. - Steroid hormone analysis in diagnosis and treatment of DSD: position paper of EU COST Action BM 1303 'DSDnet'.
Kulle A, Krone N, Holterhus PM, Schuler G, Greaves RF, Juul A, de Rijke YB, Hartmann MF, Saba A, Hiort O, Wudy SA; EU COST Action. Kulle A, et al. Eur J Endocrinol. 2017 May;176(5):P1-P9. doi: 10.1530/EJE-16-0953. Epub 2017 Feb 10. Eur J Endocrinol. 2017. PMID: 28188242 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous