Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda. Protein association and disassociation reactions responsible for localized initiation of replication - PubMed (original) (raw)
. 1989 Jun 25;264(18):10719-25.
Affiliations
- PMID: 2525130
Free article
Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda. Protein association and disassociation reactions responsible for localized initiation of replication
M Dodson et al. J Biol Chem. 1989.
Free article
Abstract
Binding of the O protein of phage lambda to the replication origin (ori lambda) results in the formation of an organized nucleoprotein structure termed the O-some. The O-some serves to localize and initiate a six-protein sequential reaction that provides for localized unwinding of the origin region, the critical prepriming step for precise initiation of DNA replication. By the use of electron microscopy of gold-tagged antibody complexes, we have defined four stages of protein association and dissociation reactions that are involved in the prepriming pathway. First, as defined previously, O protein binds to multiple DNA sites and self-associates to form the O-some. Second, lambda P and host DnaB proteins add to the O-some to generate an O.P.DnaB.ori lambda complex. Addition of the DnaK and DnaJ proteins yields a third stage complex containing DnaK, DnaJ, O, P, and DnaB. With the addition of ATP and single-strand binding protein (SSB), the P protein is largely removed, and the DnaB acts as a helicase to generate locally unwound, SSB-coated single strand DNA. Thus, the initiation of lambda DNA replication requires ordered assembly and partial disassembly of specialized nucleoprotein structures. The disassembly activity of DnaK and DnaJ may be their general role in the heat shock response.
Similar articles
- Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.
Dodson M, Echols H, Wickner S, Alfano C, Mensa-Wilmot K, Gomes B, LeBowitz J, Roberts JD, McMacken R. Dodson M, et al. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7638-42. doi: 10.1073/pnas.83.20.7638. Proc Natl Acad Sci U S A. 1986. PMID: 3020552 Free PMC article. - The Escherichia coli chaperones involved in DNA replication.
Zylicz M. Zylicz M. Philos Trans R Soc Lond B Biol Sci. 1993 Mar 29;339(1289):271-7; discussion 277-8. doi: 10.1098/rstb.1993.0025. Philos Trans R Soc Lond B Biol Sci. 1993. PMID: 8098531 Review. - Replication of coliphage lambda DNA.
Taylor K, Wegrzyn G. Taylor K, et al. FEMS Microbiol Rev. 1995 Aug;17(1-2):109-19. doi: 10.1111/j.1574-6976.1995.tb00192.x. FEMS Microbiol Rev. 1995. PMID: 7669336 Review.
Cited by
- Convergent evolution in two bacterial replicative helicase loaders.
Chase J, Berger J, Jeruzalmi D. Chase J, et al. Trends Biochem Sci. 2022 Jul;47(7):620-630. doi: 10.1016/j.tibs.2022.02.005. Epub 2022 Mar 26. Trends Biochem Sci. 2022. PMID: 35351361 Free PMC article. Review. - The Essential, Ubiquitous Single-Stranded DNA-Binding Proteins.
Oliveira MT, Ciesielski GL. Oliveira MT, et al. Methods Mol Biol. 2021;2281:1-21. doi: 10.1007/978-1-0716-1290-3_1. Methods Mol Biol. 2021. PMID: 33847949 - Interactions of replication initiator RctB with single- and double-stranded DNA in origin opening of Vibrio cholerae chromosome 2.
Chatterjee S, Jha JK, Ciaccia P, Venkova T, Chattoraj DK. Chatterjee S, et al. Nucleic Acids Res. 2020 Nov 4;48(19):11016-11029. doi: 10.1093/nar/gkaa826. Nucleic Acids Res. 2020. PMID: 33035310 Free PMC article. - Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.
Leng F. Leng F. Biophys Rev. 2016 Sep;8(3):197-207. doi: 10.1007/s12551-016-0204-z. Epub 2016 Jun 10. Biophys Rev. 2016. PMID: 28510223 Free PMC article. Review. - Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.
Leng F. Leng F. Biophys Rev. 2016 Nov;8(Suppl 1):123-133. doi: 10.1007/s12551-016-0239-1. Epub 2016 Nov 14. Biophys Rev. 2016. PMID: 28510217 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases