Binding sites for C-reactive protein on human monocytes are distinct from IgG Fc receptors - PubMed (original) (raw)
Binding sites for C-reactive protein on human monocytes are distinct from IgG Fc receptors
J M Zeller et al. Immunology. 1989 May.
Abstract
Previous investigations have provided evidence to suggest that C-reactive protein (CRP), an acute-phase reactant, binds to human monocytes at a membrane site that is either identical to or physically associated with IgG Fc receptors. To characterize further the relationship between monocyte CRP binding sites and IgG Fc receptors, monocytes were allowed to attach to surfaces coated with IgG or CRP and binding-site redistribution was assessed. Binding was measured by using protein-coated sheep erythrocytes (E). When attached to control (gelatin or albumin) surfaces, greater than 60% and 43% of monocytes formed rosettes with E-IgG and E-CRP, respectively. Following adherence to surface immobilized CRP, the proportion of cells binding E-IgG was unchanged; however, fewer than 20% of monocytes bound E-CRP. When attached to IgG-coated surfaces, fewer than 20% of monocytes formed rosettes with either E-IgG or E-CRP. In order to determine whether the unidirectional modulation of CRP and IgG binding sites was the result of CRP binding directly to a subclass of IgG Fc receptors, fluid-phase IgG-blocking studies were performed. When monocyte monolayers were preincubated with either monomeric or heat-aggregated IgG, a dose-dependent reduction in E-IgG binding was observed. In contrast, all concentrations of fluid-phase IgG failed to inhibit monocyte binding of E-CRP. These data indicate that CRP binds to human monocytes at a site physically associated with but distinct from IgG Fc receptors.
Similar articles
- C-reactive protein receptors on the human monocytic cell line U-937. Evidence for additional binding to Fc gamma RI.
Crowell RE, Du Clos TW, Montoya G, Heaphy E, Mold C. Crowell RE, et al. J Immunol. 1991 Nov 15;147(10):3445-51. J Immunol. 1991. PMID: 1834740 - Mechanism of inhibition of immunoglobulin G-mediated phagocytosis by monoclonal antibodies that recognize the Mac-1 antigen.
Brown EJ, Bohnsack JF, Gresham HD. Brown EJ, et al. J Clin Invest. 1988 Feb;81(2):365-75. doi: 10.1172/JCI113328. J Clin Invest. 1988. PMID: 2963020 Free PMC article. - Interactions of C-reactive protein with lymphoid cells.
James K, Baum LL, Vetter ML, Gewurz H. James K, et al. Ann N Y Acad Sci. 1982;389:274-85. doi: 10.1111/j.1749-6632.1982.tb22143.x. Ann N Y Acad Sci. 1982. PMID: 7046581 Review. - Function of C-reactive protein.
Du Clos TW. Du Clos TW. Ann Med. 2000 May;32(4):274-8. doi: 10.3109/07853890009011772. Ann Med. 2000. PMID: 10852144 Review.
Cited by
- C-Reactive Protein: Pathophysiology, Diagnosis, False Test Results and a Novel Diagnostic Algorithm for Clinicians.
Mouliou DS. Mouliou DS. Diseases. 2023 Sep 28;11(4):132. doi: 10.3390/diseases11040132. Diseases. 2023. PMID: 37873776 Free PMC article. Review. - Expression, Role, and Regulation of Neutrophil Fcγ Receptors.
Wang Y, Jönsson F. Wang Y, et al. Front Immunol. 2019 Aug 27;10:1958. doi: 10.3389/fimmu.2019.01958. eCollection 2019. Front Immunol. 2019. PMID: 31507592 Free PMC article. Review. - The role of genetic variants in CRP in radiographic severity in African Americans with early and established rheumatoid arthritis.
Danila MI, Westfall AO, Raman K, Chen L, Reynolds RJ, Hughes LB, Arnett DK, McGwin G, Szalai AJ, van der Heijde DM, Conn D, Callahan LF, Moreland LW, Bridges SL Jr. Danila MI, et al. Genes Immun. 2015 Oct;16(7):446-51. doi: 10.1038/gene.2015.24. Epub 2015 Jul 30. Genes Immun. 2015. PMID: 26226010 Free PMC article. - C-reactive protein: an activator of innate immunity and a modulator of adaptive immunity.
Du Clos TW, Mold C. Du Clos TW, et al. Immunol Res. 2004;30(3):261-77. doi: 10.1385/IR:30:3:261. Immunol Res. 2004. PMID: 15531769 Review. - C-reactive protein, inflammation, and innate immunity.
Mortensen RF. Mortensen RF. Immunol Res. 2001;24(2):163-76. doi: 10.1385/IR:24:2:163. Immunol Res. 2001. PMID: 11594454 Review.
References
- Clin Exp Immunol. 1986 Mar;63(3):718-27 - PubMed
- J Exp Med. 1986 Apr 1;163(4):826-36 - PubMed
- J Leukoc Biol. 1986 Dec;40(6):769-83 - PubMed
- FEBS Lett. 1987 Jan 26;211(2):165-8 - PubMed
- Inflammation. 1988 Aug;12(4):391-405 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous