Modulation of oligodendrocyte generation during a critical temporal window after NG2 cell division - PubMed (original) (raw)

Figure 1. Temporal dynamics of oligodendrocyte differentiation after NG2 cell division in vivo

(a) Experimental protocol for EDU pulse-chase labeling in P8 and P21 NG2creER:YFP mice. (b) Labeling for YFP, EDU, and CC1 at 1 and 4 days after EDU injection. Arrows: YFP+EDU+CC1- cells, Arrowheads: YFP+EDU+CC1+ cells. (c) YFP+EDU+ cortical cell pairs immunostained for NG2 or CC1. Scale Bars in b–e 25μm. (d) The proportion of symmetric CC1– (two CC1–cells), symmetric CC1+ (two CC1+ cells), or asymmetric (one CC1+ and one CC1–cell) divisions in the cortex (CTX) and corpus callosum (CC) 2, 3 and 4 days after EDU injection. (e) Percentage of YFP+EDU+ cells that were CC1+ at the indicated days after EDU injection at P8. Cortex 0→2 *_p_=0.0002, t=5.554, 0→3 *p<0.0001, t=12.92, 0→4 *p<0.0001, t=13.04, 1→2 *_p_=0.0008, t=4.946, 1→3 *p<0.0001, t=12.32, 1→4 *p<0.0001, t=12.43, 2→3 *p<0.0001, t=7.370, 2→4 *p<0.0001, t=7.483; Corpus Callosum 0→2 *p<0.0001, t=9.639, 0→3 *p<0.0001, t=17.34, 0→4 *p<0.0001, t=17.73, 1→2 *p<0.0001, t=9.800, 1→3 *p<0.0001, t=17.50, 1→4 *p<0.0001, t=17.89, 2→3 *p<0.0001, t=7.701, 2→4 *p<0.0001, t=8.094. (f) Percentage of YFP+EDU+ cells that were CC1+ after EDU injection at P21. Cortex 1→4 *_p_=0.0269, t=3.427, 1→6 *p<0.0001, t=6.616, 1→8 p<0.0001, t=7.277, 1→10 *p<0.0001, t=7.160, 2→4 *_p_=0.0281, t=3.410, 2→6 *p<0.0001, t=6.597, 2→8 *p<0.0001, t=7.261, 2→10 *p<0.0001, t=7.143, 4→8 *_p_=0.0086, t=3.850, 4→10*_p_=0.0119, t=3.733; Corpus Callosum 1→4 *p<0.0001, t=6.868, 1→6 *p<0.0001, t=11.77, 1→8 *p<0.0001, t=11.73, 1→10 *p<0.0001, t=13.09, 2→4 *p<0.0001, t=5.619, 2→6 *p<0.0001, t=10.33, 2→8 *p<0.0001, t=10.48, 2→10 *p<0.0001, t=11.84, 4→6 *_p_=0.0088, t=3.843, 4→8 *_p_=0.0005, t=4.862, 4→10 *p<0.0001, t=6.226, 6→10 *_p_=0.0333, t=3.346. n=3 mice per group and genotype. Error Bars = SD. (*two-way ANOVA, Bonferroni post test).