Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA - PubMed (original) (raw)
Comparative Study
Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA
D Scherly et al. EMBO J. 1989.
Abstract
The interaction between the U1 snRNP-specific U1 A protein and U1 snRNA has been analysed. The binding site for the protein on the RNA is shown to be in hairpin II, which extends from positions 48 to 91 in the RNA. Within this hairpin the evolutionarily conserved loop sequence is crucial for interaction with U1 A protein. U1 A protein can also bind the loop sequence when it is part of an artificial RNA which cannot form a stable hairpin structure. The region of the protein required to bind to U1 snRNA consists of a conserved 80 amino acid motif, previously identified in many ribonucleoprotein (RNP) proteins, together with (maximally) 11 N-terminal and 10 C-terminal flanking amino acids. Point mutations introduced into two of the most highly conserved regions of this motif abolish RNA binding. U1 snRNA mutants from which the U1 A binding site has been deleted are shown to be capable of assembly into RNP particles which are immunoprecipitable by patient antisera which recognize U1 A protein. The role of RNA-protein and protein-protein interactions in U snRNP assembly are discussed.
Similar articles
- Loop I of U1 small nuclear RNA is the only essential RNA sequence for binding of specific U1 small nuclear ribonucleoprotein particle proteins.
Hamm J, van Santen VL, Spritz RA, Mattaj IW. Hamm J, et al. Mol Cell Biol. 1988 Nov;8(11):4787-91. doi: 10.1128/mcb.8.11.4787-4791.1988. Mol Cell Biol. 1988. PMID: 2974920 Free PMC article. - Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing.
Hamm J, Dathan NA, Scherly D, Mattaj IW. Hamm J, et al. EMBO J. 1990 Apr;9(4):1237-44. doi: 10.1002/j.1460-2075.1990.tb08231.x. EMBO J. 1990. PMID: 2138978 Free PMC article. - Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA.
Surowy CS, van Santen VL, Scheib-Wixted SM, Spritz RA. Surowy CS, et al. Mol Cell Biol. 1989 Oct;9(10):4179-86. doi: 10.1128/mcb.9.10.4179-4186.1989. Mol Cell Biol. 1989. PMID: 2531275 Free PMC article. - Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA.
Lutz-Freyermuth C, Query CC, Keene JD. Lutz-Freyermuth C, et al. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6393-7. doi: 10.1073/pnas.87.16.6393. Proc Natl Acad Sci U S A. 1990. PMID: 1696729 Free PMC article.
Cited by
- RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins.
Hoffman DW, Query CC, Golden BL, White SW, Keene JD. Hoffman DW, et al. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2495-9. doi: 10.1073/pnas.88.6.2495. Proc Natl Acad Sci U S A. 1991. PMID: 1826055 Free PMC article. - A weak interaction between the U2A' protein and U2 snRNA helps to stabilize their complex with the U2B" protein.
Boelens W, Scherly D, Beijer RP, Jansen EJ, Dathan NA, Mattaj IW, van Venrooij WJ. Boelens W, et al. Nucleic Acids Res. 1991 Feb 11;19(3):455-60. doi: 10.1093/nar/19.3.455. Nucleic Acids Res. 1991. PMID: 1826350 Free PMC article. - Evolutionary conservation of the spliceosomal protein, U2B''.
Simpson GG, Vaux P, Clark G, Waugh R, Beggs JD, Brown JW. Simpson GG, et al. Nucleic Acids Res. 1991 Oct 11;19(19):5213-7. doi: 10.1093/nar/19.19.5213. Nucleic Acids Res. 1991. PMID: 1833724 Free PMC article. - The U1-snRNP complex: structural properties relating to autoimmune pathogenesis in rheumatic diseases.
Kattah NH, Kattah MG, Utz PJ. Kattah NH, et al. Immunol Rev. 2010 Jan;233(1):126-45. doi: 10.1111/j.0105-2896.2009.00863.x. Immunol Rev. 2010. PMID: 20192997 Free PMC article. Review. - The linked units of 5S rDNA and U1 snDNA of razor shells (Mollusca: Bivalvia: Pharidae).
Vierna J, Jensen KT, Martínez-Lage A, González-Tizón AM. Vierna J, et al. Heredity (Edinb). 2011 Aug;107(2):127-42. doi: 10.1038/hdy.2010.174. Epub 2011 Mar 2. Heredity (Edinb). 2011. PMID: 21364693 Free PMC article.
References
- EMBO J. 1987 Feb;6(2):469-76 - PubMed
- Proc Natl Acad Sci U S A. 1987 Dec;84(24):9094-8 - PubMed
- Mol Cell Biol. 1987 Sep;7(9):3268-76 - PubMed
- J Immunol. 1988 Jan 1;140(1):311-7 - PubMed
- Nucleic Acids Res. 1987 Dec 23;15(24):10373-91 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources