Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents - PubMed (original) (raw)

Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents

Charles Windson Isidoro Haminiuk et al. J Food Sci Technol. 2014 Oct.

Abstract

The recovery of phenolic compounds of Eugenia pyriformis using different solvents was investigated in this study. The compounds were identified and quantified by reverse-phase high-performance liquid chromatography coupled with ultraviolet-visible diode-array detector (RP-HPLC-DAD/UV-vis). Absolute methanol was the most effective extraction agent of phenolic acids and flavonols (588.31 mg/Kg) from Eugenia pyriformis, although similar results (p ≤ 0.05) were observed using methanol/water (1:1 ratio). Our results clearly showed that higher contents of phenolic compounds were not obtained either with the most or the least polar solvents used. Several phenolic compounds were identified in the samples whereas gallic acid and quercetin were the major compounds recovered.

Keywords: Eugenia pyriformis; Extraction; Flavonols; HPLC; Phenolic acids; Solvents.

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Figures

Fig. 1

Fig. 1

Chromatogram of phenolic compounds standards at 280 nm

Fig. 2

Fig. 2

Chromatografic profile of Eugenia pyriformis at 280 nm with absolute methanol as extraction agent

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