Characterization of the IL-2-receptor on rheumatoid arthritis synovial fluid T cells - PubMed (original) (raw)
Characterization of the IL-2-receptor on rheumatoid arthritis synovial fluid T cells
M J Dauphinée et al. J Autoimmun. 1989 Dec.
Abstract
We studied the hypoproliferative response of synovial fluid (SF) T cells in rheumatoid arthritis (RA) using a mitogenic monoclonal antibody (MoAb) specific for the T-cell antigen receptor-associated CD3 complex. RASF T cells are defective in their proliferative response and in the induction of the Tac (p55) component of the IL-2-receptor (IL-2-R) when stimulated with anti-CD3 monoclonal antibody (MoAb). However, fresh RASF T cells bear demonstrable IL-2-R in cross-linking experiments which are not seen in unstimulated peripheral blood (PB). These receptors are functional since RASF T cells proliferate in response to recombinant IL-2 (rIL-2) better than fresh PB T cells from either normal or RA patients. Scatchard analysis indicates increased (4-fold) numbers of high affinity IL-2-R on (phytohaemagglutinin) PHA-activated RASF T cells as compared with comparably activated RAPB T cells. Phorbol myristate acetate (PMA) induces Tac antigen expression in RASF but does not lead to proliferation. The hyporesponsiveness of RASF T cells does not appear to result from lack of IL-2-R, lack of IL-2-R inducibility, or proliferative potential.
Similar articles
- A functional study of purified CD4+ and CD8+ cells isolated from synovial fluid of patients with rheumatoid arthritis and other arthritides.
Hovdenes J, Gaudernack G, Kvien TK, Egeland T, Mellbye OJ. Hovdenes J, et al. Scand J Immunol. 1989 Jun;29(6):641-9. doi: 10.1111/j.1365-3083.1989.tb01168.x. Scand J Immunol. 1989. PMID: 2500703 - The CD69 activation pathway in rheumatoid arthritis synovial fluid T cells.
Hernández-García C, Fernández-Gutiérrez B, Morado IC, Bañares AA, Jover JA. Hernández-García C, et al. Arthritis Rheum. 1996 Aug;39(8):1277-86. doi: 10.1002/art.1780390803. Arthritis Rheum. 1996. PMID: 8702434 - Down-regulation of the T cell receptor CD3 zeta chain in rheumatoid arthritis (RA) and its influence on T cell responsiveness.
Berg L, Rönnelid J, Klareskog L, Bucht A. Berg L, et al. Clin Exp Immunol. 2000 Apr;120(1):174-82. doi: 10.1046/j.1365-2249.2000.01180.x. Clin Exp Immunol. 2000. PMID: 10759780 Free PMC article. - Defective induction of T-cell help and natural killing following anti-CD3 stimulation of autoimmune lymphocytes.
Tovar Z, Dauphinée MJ, Talal N. Tovar Z, et al. J Autoimmun. 1988 Aug;1(4):327-37. doi: 10.1016/0896-8411(88)90003-0. J Autoimmun. 1988. PMID: 2978115 - The T cell system in rheumatoid arthritis: activated or defective?
Kitas GD, Salmon M, Allan IM, Bacon PA. Kitas GD, et al. Scand J Rheumatol Suppl. 1988;76:161-73. doi: 10.3109/03009748809102966. Scand J Rheumatol Suppl. 1988. PMID: 3075073 Review.
Cited by
- CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease.
Cao D, van Vollenhoven R, Klareskog L, Trollmo C, Malmström V. Cao D, et al. Arthritis Res Ther. 2004;6(4):R335-46. doi: 10.1186/ar1192. Epub 2004 Jun 7. Arthritis Res Ther. 2004. PMID: 15225369 Free PMC article. - Immunotherapy through the IL-2 receptor.
Verheul HA, Verveld M, Bos ES. Verheul HA, et al. Immunol Res. 1992;11(1):42-53. doi: 10.1007/BF02918607. Immunol Res. 1992. PMID: 1602182 Review. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical