Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes - PubMed (original) (raw)

. 1989 Feb 10;243(4892):800-4.

doi: 10.1126/science.2536956.

Affiliations

• PMID: 2536956
• DOI: 10.1126/science.2536956

Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes

T Matsui et al. Science. 1989.

Abstract

A genomic sequence and cloned complementary DNA has been identified for a novel receptor-like gene of the PDGF receptor/CSF1 receptor subfamily (platelet-derived growth factor receptor/colony-stimulating factor type 1 receptor). The gene recognized a 6.4-kilobase transcript that was coexpressed in normal human tissues with the 5.3-kilobase PDGF receptor messenger RNA. Introduction of complementary DNA of the novel gene into COS-1 cells led to expression of proteins that were specifically detected with antiserum directed against a predicted peptide. When the new gene was transfected into COS-1 cells, a characteristic pattern of binding of the PDGF isoforms was observed, which was different from the pattern observed with the known PDGF receptor. Tyrosine phosphorylation of the receptor in response to the PDGF isoforms was also different from the known receptor. The new PDGF receptor gene was localized to chromosome 4q11-4q12. The existence of genes encoding two PDGF receptors that interact in a distinct manner with three different PDGF isoforms likely confers considerable regulatory flexibility in the functional responses to PDGF.

PubMed Disclaimer

Similar articles

• cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor.
  Claesson-Welsh L, Eriksson A, Westermark B, Heldin CH. Claesson-Welsh L, et al. Proc Natl Acad Sci U S A. 1989 Jul;86(13):4917-21. doi: 10.1073/pnas.86.13.4917. Proc Natl Acad Sci U S A. 1989. PMID: 2544881 Free PMC article.
• Cloning and expression of a cDNA coding for the human platelet-derived growth factor receptor: evidence for more than one receptor class.
  Gronwald RG, Grant FJ, Haldeman BA, Hart CE, O'Hara PJ, Hagen FS, Ross R, Bowen-Pope DF, Murray MJ. Gronwald RG, et al. Proc Natl Acad Sci U S A. 1988 May;85(10):3435-9. doi: 10.1073/pnas.85.10.3435. Proc Natl Acad Sci U S A. 1988. PMID: 2835772 Free PMC article.
• Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors.
  Yarden Y, Escobedo JA, Kuang WJ, Yang-Feng TL, Daniel TO, Tremble PM, Chen EY, Ando ME, Harkins RN, Francke U, et al. Yarden Y, et al. Nature. 1986 Sep 18-24;323(6085):226-32. doi: 10.1038/323226a0. Nature. 1986. PMID: 3020426
• The stimulation of paracrine and autocrine mitogenic pathways by the platelet-derived growth factor receptor.
  Williams LT, Escobedo JA, Keating MT, Coughlin SR. Williams LT, et al. J Cell Physiol Suppl. 1987;Suppl 5:27-30. doi: 10.1002/jcp.1041330406. J Cell Physiol Suppl. 1987. PMID: 2824531 Review.
• Signal transduction by the platelet-derived growth factor receptor.
  Williams LT. Williams LT. Science. 1989 Mar 24;243(4898):1564-70. doi: 10.1126/science.2538922. Science. 1989. PMID: 2538922 Review.

Cited by

• The ltk gene encodes a novel receptor-type protein tyrosine kinase.
  Krolewski JJ, Dalla-Favera R. Krolewski JJ, et al. EMBO J. 1991 Oct;10(10):2911-9. doi: 10.1002/j.1460-2075.1991.tb07841.x. EMBO J. 1991. PMID: 1655406 Free PMC article.
• Diverse forms of a receptor for acidic and basic fibroblast growth factors.
  Johnson DE, Lee PL, Lu J, Williams LT. Johnson DE, et al. Mol Cell Biol. 1990 Sep;10(9):4728-36. doi: 10.1128/mcb.10.9.4728-4736.1990. Mol Cell Biol. 1990. PMID: 2167437 Free PMC article.
• A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit.
  Matthews W, Jordan CT, Gavin M, Jenkins NA, Copeland NG, Lemischka IR. Matthews W, et al. Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9026-30. doi: 10.1073/pnas.88.20.9026. Proc Natl Acad Sci U S A. 1991. PMID: 1717995 Free PMC article.
• New nucleotide sequence data on the EMBL File Server.
  [No authors listed] [No authors listed] Nucleic Acids Res. 1990 Mar 11;18(5):1321-30. doi: 10.1093/nar/18.5.1321. Nucleic Acids Res. 1990. PMID: 2320435 Free PMC article. No abstract available.
• An antibody present in normal human serum inhibits the binding of cytokines to their receptors in an in vitro system.
  Mosedale DE, Grainger DJ. Mosedale DE, et al. Biochem J. 1999 Oct 1;343 Pt 1(Pt 1):125-33. Biochem J. 1999. PMID: 10493920 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • Mouse Genome Informatics (MGI)

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal