Isolation, characterization, and nucleotide sequence of appY, a regulatory gene for growth-phase-dependent gene expression in Escherichia coli - PubMed (original) (raw)
Isolation, characterization, and nucleotide sequence of appY, a regulatory gene for growth-phase-dependent gene expression in Escherichia coli
T Atlung et al. J Bacteriol. 1989 Mar.
Abstract
A plasmid carrying a regulator gene, designated appY, was found in the screening of an Escherichia coli gene library for clones overproducing AppA, an acid phosphatase which is induced as a culture approaches the stationary phase. In cells containing multicopy plasmids carrying the appY gene, the expression of the chromosomal appY gene was stimulated 10- to 40-fold in the stationary phase and more than 100-fold during exponential growth. The appA plasmid also changed the rate of synthesis of more than 30 other proteins in a growth-phase-dependent way. The appY gene was mapped to 13 min on the E. coli genetic map. The position of the appY gene on the 4.9-kilobase HindIII fragment of the original clone was located by Tn5 mutagenesis and deletion analysis, and the nucleotide sequence of a 1.9-kilobase region containing the gene was determined. The appY gene product was identified as a weakly expressed 243-amino-acid polypeptide which contains a stretch of 20 amino acids showing very good similarity to the conserved DNA-binding domain of repressors and transcriptional activators.
Similar articles
- Role of the transcriptional activator AppY in regulation of the cyx appA operon of Escherichia coli by anaerobiosis, phosphate starvation, and growth phase.
Atlung T, Brøndsted L. Atlung T, et al. J Bacteriol. 1994 Sep;176(17):5414-22. doi: 10.1128/jb.176.17.5414-5422.1994. J Bacteriol. 1994. PMID: 8071219 Free PMC article. - Effect of growth conditions on expression of the acid phosphatase (cyx-appA) operon and the appY gene, which encodes a transcriptional activator of Escherichia coli.
Brøndsted L, Atlung T. Brøndsted L, et al. J Bacteriol. 1996 Mar;178(6):1556-64. doi: 10.1128/jb.178.6.1556-1564.1996. J Bacteriol. 1996. PMID: 8626281 Free PMC article. - The histone-like protein H-NS acts as a transcriptional repressor for expression of the anaerobic and growth phase activator AppY of Escherichia coli.
Atlung T, Sund S, Olesen K, Brøndsted L. Atlung T, et al. J Bacteriol. 1996 Jun;178(12):3418-25. doi: 10.1128/jb.178.12.3418-3425.1996. J Bacteriol. 1996. PMID: 8655536 Free PMC article. - Nucleotide sequence of plasmid NAH7 gene nahR and DNA binding of the nahR product.
You IS, Ghosal D, Gunsalus IC. You IS, et al. J Bacteriol. 1988 Dec;170(12):5409-15. doi: 10.1128/jb.170.12.5409-5415.1988. J Bacteriol. 1988. PMID: 2848005 Free PMC article.
Cited by
- The prophage-encoded transcriptional regulator AppY has pleiotropic effects on E. coli physiology.
Derdouri N, Ginet N, Denis Y, Ansaldi M, Battesti A. Derdouri N, et al. PLoS Genet. 2023 Mar 17;19(3):e1010672. doi: 10.1371/journal.pgen.1010672. eCollection 2023 Mar. PLoS Genet. 2023. PMID: 36930675 Free PMC article. - How Escherichia coli is equipped to oxidize hydrogen under different redox conditions.
Lukey MJ, Parkin A, Roessler MM, Murphy BJ, Harmer J, Palmer T, Sargent F, Armstrong FA. Lukey MJ, et al. J Biol Chem. 2010 Feb 5;285(6):3928-3938. doi: 10.1074/jbc.M109.067751. Epub 2009 Nov 16. J Biol Chem. 2010. PMID: 19917611 Free PMC article. - 6S RNA function enhances long-term cell survival.
Trotochaud AE, Wassarman KM. Trotochaud AE, et al. J Bacteriol. 2004 Aug;186(15):4978-85. doi: 10.1128/JB.186.15.4978-4985.2004. J Bacteriol. 2004. PMID: 15262935 Free PMC article. - Linkage map of Escherichia coli K-12, edition 10: the traditional map.
Berlyn MK. Berlyn MK. Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998. Microbiol Mol Biol Rev. 1998. PMID: 9729611 Free PMC article. Review. - Overlapping functions of components of a bacterial Sec-independent protein export pathway.
Sargent F, Bogsch EG, Stanley NR, Wexler M, Robinson C, Berks BC, Palmer T. Sargent F, et al. EMBO J. 1998 Jul 1;17(13):3640-50. doi: 10.1093/emboj/17.13.3640. EMBO J. 1998. PMID: 9649434 Free PMC article.
References
- J Mol Biol. 1986 Sep 5;191(1):39-58 - PubMed
- Microbiol Rev. 1986 Sep;50(3):227-43 - PubMed
- J Bacteriol. 1987 Apr;169(4):1663-9 - PubMed
- Nucleic Acids Res. 1987 May 11;15(9):3924 - PubMed
- J Bacteriol. 1987 Jul;169(7):3068-75 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases