Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells - PubMed (original) (raw)

Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells

U C Garg et al. J Clin Invest. 1989 May.

Abstract

Endothelium-derived relaxing factor has been recently identified as nitric oxide. The purpose of this study was to determine if vasodilator drugs that generate nitric oxide inhibit vascular smooth muscle mitogenesis and proliferation in culture. Three chemically dissimilar vasodilators, sodium nitroprusside, S-nitroso-N-acetylpenicillamine and isosorbide dinitrate, dose-dependently inhibited serum-induced thymidine incorporation by rat aortic smooth muscle cells. Moreover, 8-bromo-cGMP mimicked the antimitogenic effect of the nitric oxide-generating drugs. The antimitogenic effect of S-nitroso-N-acetylpenicillamine was inhibited by hemoglobin and potentiated by superoxide dismutase, supporting the view that nitric oxide was the ultimate effector. Sodium nitroprusside and S-nitroso-N-acetylpenicillamine significantly decreased the proliferation of vascular smooth muscle cells. Moreover, the inhibition of mitogenesis and proliferation was shown to be independent of cell damage, as documented by several criteria of cell viability. These results suggest that endogenous nitric oxide may function as a modulator of vascular smooth muscle cell mitogenesis and proliferation, by a cGMP-mediated mechanism.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Lab Invest. 1975 Mar;32(3):339-51 - PubMed
    1. Biochem Biophys Res Commun. 1988 Jul 15;154(1):236-44 - PubMed
    1. J Cyclic Nucleotide Res. 1979;5(3):211-24 - PubMed
    1. Circ Res. 1980 Aug;47(2):182-9 - PubMed
    1. J Pharmacol Exp Ther. 1981 Sep;218(3):739-49 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources