Dual-functional probes for sequential thiol and redox homeostasis sensing in live cells - PubMed (original) (raw)
. 2015 Jan 7;140(1):322-9.
doi: 10.1039/c4an01441c.
Affiliations
- PMID: 25406724
- DOI: 10.1039/c4an01441c
Dual-functional probes for sequential thiol and redox homeostasis sensing in live cells
Tao Ma et al. Analyst. 2015.
Abstract
A new type of resorufin-based dual-functional fluorescent probe whose fluorescence emission features are sensitive to thiol compounds and redox homeostasis was developed. Thiols-triggered nucleophilic substitution of the probes converts the nonfluorescent probe to the highly fluorescent resorufin moiety; the released resorufin not only enables fluorescence signaling specific for thiol compounds but functions as a redox indicator with sensitive colorimetric and fluorescence emission change upon redox variation. Preliminary fluorescence imaging experiments have revealed the biocompatibility of the as-prepared probes and validated their practicability for thiol sensing and redox homeostasis mapping in living cells.
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