Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein - PubMed (original) (raw)
. 1989 Jun 1;339(6223):397-8.
doi: 10.1038/339397a0.
Affiliations
- PMID: 2542798
- DOI: 10.1038/339397a0
Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein
C J Beckers et al. Nature. 1989.
Abstract
An N-ethylmaleimide-sensitive fusion protein (NSF) has been purified on the basis of its ability to catalyse vesicular transport within the Golgi stack. We report here that this same protein is required for transport from the endoplasmic reticulum to the Golgi stack in semi-intact cells. This transport process is inhibited by a monoclonal antibody against NSF. Furthermore, pretreatment of semi-intact cells with N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits transport. Addition of highly purified NSF largely restores transport from endoplasmic reticulum to Golgi. These results suggest that NSF is a general component of the transport machinery required for membrane fusion at multiple stages of the secretory pathway.
Similar articles
- A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast.
Wilson DW, Wilcox CA, Flynn GC, Chen E, Kuang WJ, Henzel WJ, Block MR, Ullrich A, Rothman JE. Wilson DW, et al. Nature. 1989 Jun 1;339(6223):355-9. doi: 10.1038/339355a0. Nature. 1989. PMID: 2657434 - Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport.
Diaz R, Mayorga LS, Weidman PJ, Rothman JE, Stahl PD. Diaz R, et al. Nature. 1989 Jun 1;339(6223):398-400. doi: 10.1038/339398a0. Nature. 1989. PMID: 2725659 - Mammalian Bet3 functions as a cytosolic factor participating in transport from the ER to the Golgi apparatus.
Loh E, Peter F, Subramaniam VN, Hong W. Loh E, et al. J Cell Sci. 2005 Mar 15;118(Pt 6):1209-22. doi: 10.1242/jcs.01723. Epub 2005 Feb 22. J Cell Sci. 2005. PMID: 15728249 - [Vesicle-mediated protein transport system].
Yoshimura T. Yoshimura T. Tanpakushitsu Kakusan Koso. 1993 May;38(7):1152-9. Tanpakushitsu Kakusan Koso. 1993. PMID: 8337386 Review. Japanese. No abstract available. - Analysis of protein transport through the Golgi in a reconstituted cell-free system.
Wattenberg BW. Wattenberg BW. J Electron Microsc Tech. 1991 Feb;17(2):150-64. doi: 10.1002/jemt.1060170204. J Electron Microsc Tech. 1991. PMID: 1901603 Review.
Cited by
- ER exit in physiology and disease.
Robinson CM, Duggan A, Forrester A. Robinson CM, et al. Front Mol Biosci. 2024 Jan 18;11:1352970. doi: 10.3389/fmolb.2024.1352970. eCollection 2024. Front Mol Biosci. 2024. PMID: 38314136 Free PMC article. Review. - LRRK2 along the Golgi and lysosome connection: a jamming situation.
Piccoli G, Volta M. Piccoli G, et al. Biochem Soc Trans. 2021 Nov 1;49(5):2063-2072. doi: 10.1042/BST20201146. Biochem Soc Trans. 2021. PMID: 34495322 Free PMC article. Review. - LRRK2 and the Endolysosomal System in Parkinson's Disease.
Erb ML, Moore DJ. Erb ML, et al. J Parkinsons Dis. 2020;10(4):1271-1291. doi: 10.3233/JPD-202138. J Parkinsons Dis. 2020. PMID: 33044192 Free PMC article. Review. - Porcine Epidemic Diarrhea Virus ORF3 Protein Is Transported through the Exocytic Pathway.
Si F, Chen B, Hu X, Yu R, Dong S, Wang R, Li Z. Si F, et al. J Virol. 2020 Aug 17;94(17):e00808-20. doi: 10.1128/JVI.00808-20. Print 2020 Aug 17. J Virol. 2020. PMID: 32554695 Free PMC article. - A family of PIKFYVE inhibitors with therapeutic potential against autophagy-dependent cancer cells disrupt multiple events in lysosome homeostasis.
Sharma G, Guardia CM, Roy A, Vassilev A, Saric A, Griner LN, Marugan J, Ferrer M, Bonifacino JS, DePamphilis ML. Sharma G, et al. Autophagy. 2019 Oct;15(10):1694-1718. doi: 10.1080/15548627.2019.1586257. Epub 2019 Mar 8. Autophagy. 2019. PMID: 30806145 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources